Article Figures & Data
Figures
- FIG. 1.
Chemical structure of EC, the compound used in this study.
- FIG. 2.
Inhibitory effects of EC complex 1 (at 100 and 20 μg/ml) and the solvent DMSO (at 0.1 and 0.02%) on the growth of L. major and L. infantum after 2, 24, and 48 h of contact. Percentages were calculated relative to the growth of promastigotes in medium, considered as 100%.
- FIG. 3.
Inhibitory effects of EC complex 1 and DMSO (A) and of EC complex 2 and DIMEB (B) on the growth of E. vannus strain TB6.
- FIG. 4.
Effects of the two Euplotes strains on the viability of L. major and L. infantum. (A) E. crassus strain SSt22 (euplotin-producing strain); (B) E. vannus strain TB6 (non-euplotin-producing strain). The Euplotes strains remained viable for the duration of the experiment.
Tables
- TABLE 1.
Inhibitory and killing activities of EC against L. major at different times
EC complex % Activitya of EC at the indicated time and concn against L. major After 2 h After 24 h After 48 h 100 μg/ml 20 μg/ml 100 μg/ml 20 μg/ml 100 μg/ml 20 μg/ml A B A B A B A B A B A B 1 94 92 66 60 100 100 97 92 100 100 56 51 2 83 78 42 39 95 92 72 69 100 100 90 85 ↵ a A, inhibitory activity, B, killing activity.
- TABLE 2.
Antimicrobial activities of the two complexes of EC and the two solvents against different microorganismsa
Microorganism % of vital microorganisms after treatment with: Complex 1 DMSO Complex 2 DIMEB 2 h 24 h 2 h 24 h 2 h 24 h 2 h 24 h Gram-positive bacteria Micrococcus luteus ATCC 9341 100 100 100 100 100 100 100 100 Staphylococcus aureus ATCC 6538 100 100 100 100 100 100 100 100 Streptococcus pyogenes ATCC 11434 40 4 85 15 100 66 100 100 Streptococcus pyogenes ATCC 8668 100 10 100 80 100 70 100 100 Gram-negative bacteria Escherichia coli HB101 60 48 65 90 100 80 100 92 Proteus vulgaris N9 100 100 100 100 100 100 100 100 Pseudomonas aeruginosa ATCC 15442 95 85 95 90 97 72 100 90 Burkholderia cepacia ATCC 17759 38 6 55 5 95 90 100 100 Burkholderia cepacia 1/2000 7 1 24 5 90 90 100 100 Yeast, Candida albicans 6 1 7 82 25 60 70 94 90 ↵ a EC was used at 100 μg/ml. DMSO was used as a solvent with EC complex 1, and DIMEB was used as a solvent with EC complex 2.
