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Mechanisms of Resistance

TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes

Laurent Poirel, Hedi Mammeri, Patrice Nordmann
Laurent Poirel
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France
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Hedi Mammeri
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France
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Patrice Nordmann
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France
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  • For correspondence: nordmann.patrice@bct.ap-hop-paris.fr
DOI: 10.1128/AAC.48.12.4528-4531.2004
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ABSTRACT

Enterobacter aerogenes clinical isolate LOR was resistant to penicillins and ceftazidime but susceptible to cefuroxime, cephalothin, cefoxitin, cefotaxime, ceftriaxone, and cefepime. PCR and cloning experiments from this strain identified a novel TEM-type β-lactamase (TEM-121) differing by five amino acid substitutions from β-lactamase TEM-2 (Glu104Lys, Arg164Ser, Ala237Thr, Glu240Lys, and Arg244Ser) and by only one amino acid change from the extended-spectrum β-lactamase (ESBL) TEM-24 (Arg244Ser), with the last substitution also being identified in the inhibitor-resistant β-lactamase IRT-2. Kinetic parameters indicated that TEM-121 hydrolyzed ceftazidime and aztreonam (like TEM-24) and was inhibited weakly by clavulanic acid and strongly by tazobactam. Thus, TEM-121 is a novel complex mutant TEM β-lactamase (CMT-4) combining the kinetic properties of an ESBL and an inhibitor-resistant TEM enzyme.

  • Copyright © 2004 American Society for Microbiology
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TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes
Laurent Poirel, Hedi Mammeri, Patrice Nordmann
Antimicrobial Agents and Chemotherapy Nov 2004, 48 (12) 4528-4531; DOI: 10.1128/AAC.48.12.4528-4531.2004

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TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes
Laurent Poirel, Hedi Mammeri, Patrice Nordmann
Antimicrobial Agents and Chemotherapy Nov 2004, 48 (12) 4528-4531; DOI: 10.1128/AAC.48.12.4528-4531.2004
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KEYWORDS

ceftazidime
cephalosporins
Enterobacter aerogenes
beta-lactamases

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