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Mechanisms of Resistance

TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes

Laurent Poirel, Hedi Mammeri, Patrice Nordmann
Laurent Poirel
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France
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Hedi Mammeri
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France
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Patrice Nordmann
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France
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  • For correspondence: nordmann.patrice@bct.ap-hop-paris.fr
DOI: 10.1128/AAC.48.12.4528-4531.2004
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Tables

  • TABLE 1.

    MICs of β-lactams for E. aerogenes LOR and CF23.04 isolates and for recombinant E. coli DH10B(pBK-TEM-121), DH10B(pBK-TEM-24), and DH10B

    β-LactamaMIC (μg/ml) for the following isolateb:
    E. aerogenes LORE. coli DH10B(pBK-TEM-121)E. aerogenes CF24.03E. coli DH10B(pBK-TEM-24)E. coli DH10B
    Amoxicillin>512>512>512>5122
    Amoxicillin-CLA163251242
    Ticarcillin>512>512>5125121
    Ticarcillin-CLA128256128321
    Piperacillin32641281281
    Piperacillin-TZB481681
    Cephalothin816>512>5124
    Cefoxitin4212822
    Cefuroxime88>5122560.5
    Ceftriaxone<0.06<0.06328<0.06
    Cefotaxime<0.06<0.06328<0.06
    Ceftazidime128256512256<0.06
    Aztreonam8165122560.06
    Cefepime24440.06
    Cefpirome24440.06
    Imipenem0.060.060.060.060.06
    • ↵ a CLA, clavulanic acid at 2 μg/ml; TZB, tazobactam at 4 μg/ml.

    • ↵ b E. aerogenes isolate LOR produced β-lactamase TEM-121, whereas E. aerogenes CF24.03 produced TEM-24 and AmpC-type β-lactamases. Recombinant E. coli DH10B(pBK-TEM-121) and E. coli DH10B(pBK-TEM-24) produced β-lactamases TEM-121 and TEM-24, respectively.

  • TABLE 2.

    Kinetic parameters of β-lactamases TEM-121 and TEM-24a

    β-LactamTEM-121TEM-24
    kcat (s−1)Km (μM)kcat/Km (mM−1 · s−1)kcat (s−1)Km (μM)kcat/Km (mM−1 · s−1)
    Benzylpenicillin25151,7002092,200
    Amoxicillin1050020655110
    Ticarcillin10751401060160
    Piperacillin715140661,000
    Cephaloridine7027026090901,000
    Cephalothin204205030301,000
    Cefuroxime>5>700145330140
    Ceftriaxone>2>500410101,000
    Cefotaxime0.5430108.525340
    Ceftazidime40150270120180670
    Cefepime640150990100
    Cefpirome303608045310145
    Aztreonam6.580801655290
    • ↵ a Values are the means of results from at least three independent experiments.

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TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes
Laurent Poirel, Hedi Mammeri, Patrice Nordmann
Antimicrobial Agents and Chemotherapy Nov 2004, 48 (12) 4528-4531; DOI: 10.1128/AAC.48.12.4528-4531.2004

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TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes
Laurent Poirel, Hedi Mammeri, Patrice Nordmann
Antimicrobial Agents and Chemotherapy Nov 2004, 48 (12) 4528-4531; DOI: 10.1128/AAC.48.12.4528-4531.2004
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KEYWORDS

ceftazidime
cephalosporins
Enterobacter aerogenes
beta-lactamases

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