Article Figures & Data
Tables
- TABLE 1.
Primers used for DNA amplification
Primer 5′ → 3′ nucleotide sequence Reference nalC1 TCA ACC CTA ACG AGA AAC GCT This study nalC2 TCC ACC TCA CCG AAC TGC This study mexA-1 CGA CCA GGC CGT GAG CAA GCA GC Dumas et al. (submitted) mexA-2 GGA GAC CTT CGC CGC GTT GTC GC Dumas et al. (submitted) mexX-1 TGA AGG CGG CCC TGG ACA TCA GC Dumas et al. (submitted) mexX-2 GAT CTG CTC GAC GCG GGT CAG CG Dumas et al. (submitted) PA3720-1 TCG CCC TGG TCT ATC CGC CGC TC This study PA3720-2 CCG CTC AGC AGT GCC TTC GCC AT This study rpsL-1 GCA ACT ATC AAC CAG CTG GTG Dumas et al. (submitted) rpsL-2 GCT GTG CTC TTG CAG GTT GTG Dumas et al. (submitted) - TABLE 2.
Susceptibilities of P. aeruginosa strains to antimicrobial agents
Strain Genotype Aminoglycoside- modifying enzyme MICa (μg/ml) nal agr Tic Caz Fep Atm Amk Tob Apr For Cip PAO1 16 2 2 4 4 0.5 16 16 0.12 Mut-Gr1 agrZ 16 2 4 4 8 1 64 64 0.5 PT629 nalB 64 4 4 16 4 0.5 16 16 0.25 ATM4 nalB agrZ 64 4 16 16 8 1 ND ND 1 WL22 nalB agrZ More than 2b 128 8 16 32 128 128 64 128 64 WL24 nalD agrW ANT(2′′)-I 128 8 16 32 16 64 64 128 64 1113 nalD agrZ 64 4 8 16 8 1 32 128 0.5 1217 nalD agrZ More than 2 64 4 16 16 64 32 128 128 64 1237 nalB agrZ AAC(6′)-II 64 4 8 16 16 128 64 128 16 1250 nalC agrZ 64 4 8 16 8 1 16 32 0.5 1562 nalD agrZ More than 2 64 4 16 16 128 64 128 128 32 1727 nalB nalC agrW 128 8 16 32 32 2 64 128 2 1738 nalC agrW 64 2 8 16 8 1 32 128 0.5 2085 nalB agrZ AAC(3)-VI 32 2 8 16 16 4 64 128 8 2151 nalB agrZ 32 2 8 16 8 1 64 128 32 2172 nalC agrZ 64 4 8 16 8 1 64 128 1 ↵ a Tic, ticarcillin; Caz, ceftazidime; Fep, cefepime; Atm, aztreonam; Amk, amikacin; Tob, tobramycin; Apr, apramycin; For, fortimicin; Cip, ciprofloxacin. Values in boldface are at least fourfold higher than that for PAO1. ND, not determined.
↵ b Complex susceptibility profile suggesting the production of two or more different modifying enzymes.
- TABLE 3.
Genetic analysis of MexAB-OprM- and MexXY-overproducing strains of P. aeruginosa
Strain Nucleotide sequencing dataa Mean gene expressionb mexZc mexRd PA3721e Vicinity of PA3721 mexX mexA PA3720 PAO1 1.0 1.0 1.0 PT629 (nalB) ND 4 bp deleted at posi- tion 363 0.6 3.6 1.71 ATM4 (nalB agrZ) CAG→TAG (C307T) GCA→CCA (A66P) 482 6.5 0.11 WL22 48 44, 126 71, 153 G−73→A, A+13→T 168 9.2 2.9 WL24 71, 209 22.0 5.1 0.2 1113 GCC→-CC (A35−), ATG→ AT-(M34−) 126 71, 209 T−2→C, C inserted (−25) 29.6 4.4 5.5 1217 144, GAG→-AG (E157−) 71 98.5 6.0 0.5 1237 48, CAG→TAG (Q10Z) 44, 126 71, 153, 209, TCA→GCA (S46A) A+13→T 272 7.7 3.7 1250 GGT→G--(G46−), GCG→- CG (A47−) 500 bp deleted 312 17.9 147 1562 144 71 129 8.5 1.7 1727 7-bp insertion between 355 and 361 71, GAT→GAA (D76E) 300 34.8 9.9 1738 71, 209, CTG→CCG (L61P) 82.2 11.7 224 2085 48 44, 126 71, 153 T−2→C 204 8.7 5.8 2151 GTC→GCC (V44A) 44, 126 71, 153, 209 T−2→C, A+13→T 110 2.6 6.7 2172 GGT→AGT (G46S) 71, ATG→ACG (M151T) 95.4 6.1 194 ↵ a Nucleotide differences compared with the PAO1 genome (www.pseudomonas.com ).
↵ b Relative to PAO1; mean values from two independent experiments.
↵ c 48, substitution GTC→GCC (V48→A); 144, GCG→GTG (V144→A).
↵ d 44, substitution AAG→ATG (K44→M); 126, GTG→GAG (V126→E).
↵ e 71, substitution GGG→GAG (G71→E); 153, GAG→CAG (E153→Q); 209, AGC→CGC (S209→R).
