DOI: 10.1128/AAC.48.8.3203-3206.2004
Article Figures & Data
Figures
- FIG. 1.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of outer membrane proteins with protein standards of 97, 66, 49, and 30 kDa (lane A), K. pneumoniae C2 (lane B), K. pneumoniae 002(pMG251) (lane C), the K. pneumoniae 002(pMG251) ertapenem-resistant derivative for which the MIC was 4 μg/ml (lane D), and the K. pneumoniae 002(pMG251) ertapenem-resistant derivative for which the MIC was 128 μg/ml (lane E).
Tables
- TABLE 1.
Susceptibilities of porin-deficient K. pneumoniae strain C2 containing various plasmid-mediated β-lactamases
Enzyme group β-Lactamase Plasmid MIC (μg/ml) Cefepime Cefotaxime Cefoxitin Ceftazidime Ertapenem Imipenem Meropenem None 0.5 0.5 64 0.5 1 1 0.25 1 ACC-1 pSLK54 8 128 ≥1,024 ≥256 32 8 4 ACT-1 pMG251 2 128 ≥1,024 64 ≥128 64 16 CMY-2 pMG250 4 128 ≥1,024 128 32 32 8 DHA-1 pMG247 4 256 ≥1,024 ≥256 128 64 8 FOX-1 pGL3a 16 ≥256 ≥1,024 ≥256 128 64 16 FOX-3 p1734 2 32 ≥1,024 128 8 2 2 FOX-5 pMG252 4 64 ≥1,024 128 8 2 1 LAT-1 pHP15 8 ≥256 ≥1,024 ≥256 64 32 16 MIR-1 pMG233 2 64 ≥1,024 32 32 32 8 MOX-1 pRMOX-1 4 32 ≥1,024 64 32 8 8 MOX-2 pKOL 8 64 ≥1,024 128 16 2 2 2b TEM-1 R1 0.5 0.5 64 0.5 2 1 0.5 TEM-2 RP1 4 0.5 64 1 8 2 2 2be TEM-3 pCFF04 32 128 64 128 8 2 1 TEM-4 pUD16 32 128 64 32 16 2 4 TEM-5 pCFF14 16 16 64 256 16 4 1 TEM-6 pMG226 16 4 16 ≥256 32 1 1 TEM-7 pIF100 32 2 64 64 16 2 2 TEM-8 Plasmid from E. coli CF804 32 16 64 ≥256 16 2 0.5 TEM-9 pMG228 32 16 64 ≥256 32 4 0.5 TEM-10 pMG223 16 4 64 ≥256 16 2 0.5 TEM-11 pMG244 16 2 64 64 16 2 4 TEM-12 pMG224 16 2 64 64 16 2 0.5 TEM-15 pMG274 16 64 64 ≥256 8 2 2 TEM-16 Plasmid from K. pneumoniae CF1304 16 4 64 ≥256 8 1 0.5 TEM-19 pMG289 8 64 32 64 16 4 1 TEM-20 pUD30 64 32 64 16 4 2 2 TEM-21 pUD31 16 128 64 256 16 4 0.5 TEM-22 Plasmid from E. coli HB101 TEM-22 8 64 64 ≥256 16 2 0.25 TEM-24 Plasmid from K. pneumoniae CF1104 16 32 64 ≥256 8 0.5 0.5 TEM-25 Plasmid from E. coli CF1609 16 32 64 32 16 4 0.25 TEM-26 pMG225 32 4 64 ≥256 8 1 0.25 TEM-52 pMG276 8 32 64 64 8 4 0.5 TEM-61 pMG290 8 4 16 ≥256 16 4 2 TEM-71 pMG259 1 0.5 64 256 8 4 0.125 TEM-88 pMG272 8 32 64 64 16 2 0.5 2b SHV-1 R1010 0.5 0.5 64 0.5 2 0.5 0.5 2be SHV-2 pMG258 16 4 64 ≥256 8 1 0.25 SHV-3 pUD18 32 64 64 ≥256 4 2 1 SHV-4 pUD21 8 16 64 256 4 0.5 0.25 SHV-5 pAFF2 16 32 64 ≥256 8 2 2 SHV-6 pSLH47 1 0.5 64 32 8 0.5 1 SHV-12 pMG242 4 4 32 ≥256 4 2 2 SHV-18 pMG266 4 8 64 64 8 1 2 CTX-M-5 pCLL3417a 128 ≥256 64 4 8 2 1 CTX-M-14 pMG267 32 32 64 8 8 2 2 2b HMS-1 R997 0.5 0.5 64 1 2 0.5 1 2c PSE-1 pMG217 0.5 0.5 64 4 2 1 0.5 PSE-4 pUZ8::Tn1405 0.5 0.5 64 0.5 2 1 2 CARB-3 pUZ8::Tn1408 1 0.5 64 0.5 1 1 1 CARB-4 pUZ8::Tn1413 0.5 0.25 32 0.5 1 1 2 SAR-1 pUK657 0.5 0.5 64 0.5 2 1 0.5 2d OXA-1 RGN238 4 0.5 64 0.5 2 1 2 OXA-2 R46 0.5 0.5 64 16 32 2 1 OXA-3 R55 0.25 0.25 16 2 1 0.5 2 OXA-4 pMG203 1 0.5 64 0.5 1 1 0.25 OXA-5 pMG54 0.5 0.5 64 0.5 1 1 0.25 OXA-7 pMG202 1 1 32 1 1 1 2 OXA-10 pUZ8::Tn1404 0.5 0.5 64 0.5 2 1 2 LCR-1 pUZ8::Tn1412 0.5 0.5 64 0.5 2 0.5 1 2f KPC-1 pBR322-catI-blaKPC-1a 128 128 128 64 ≥128 ≥128 ≥128 3 VIM-2 pNOR2001a 4 64 128 32 64 64 128 ↵a Multicopy recombinant plasmid.
- TABLE 2.
Susceptibilities of K. pneumoniae 002(pMG251) and its derivatives to β-lactams
Strain MIC (μg/ml) Cefepime Cefotaxime Cefoxitin Ceftazidime Ertapenem Imipenem Meropenem 002(pMG251) 0.125 2 512 8 0.5 2 0.125 002(pMG251) Ertapenemr MIC 4a 1 32 ≥1,024 16 4 16 1 002(pMG251) Ertapenemr MIC 4 carrying pQE7Kb 0.5 16 ≥1,024 8 2 2 0.5 002(pMG251) Ertapenemr MIC 4 carrying pSHA25Kc 0.125 2 512 8 0.25 2 0.125 002(pMG251) Ertapenemr MIC 128d 2 32 ≥1,024 ≥32 128 ≥128 16 002(pMG251) Ertapenemr MIC 128 carrying pQE7K 1 32 ≥1,024 ≥32 16 8 2 002(pMG251) Ertapenemr MIC 128 carrying pSHA25K 0.06 1 512 8 0.25 2 0.125
