Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Antimicrobial Agents and Chemotherapy
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mechanisms of Resistance

Role of β-Lactamases and Porins in Resistance to Ertapenem and Other β-Lactams in Klebsiella pneumoniae

George A. Jacoby, Debra M. Mills, Nancy Chow
George A. Jacoby
Lahey Clinic, Burlington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: george.a.jacoby@lahey.org
Debra M. Mills
Lahey Clinic, Burlington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nancy Chow
Lahey Clinic, Burlington, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/AAC.48.8.3203-3206.2004
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of outer membrane proteins with protein standards of 97, 66, 49, and 30 kDa (lane A), K. pneumoniae C2 (lane B), K. pneumoniae 002(pMG251) (lane C), the K. pneumoniae 002(pMG251) ertapenem-resistant derivative for which the MIC was 4 μg/ml (lane D), and the K. pneumoniae 002(pMG251) ertapenem-resistant derivative for which the MIC was 128 μg/ml (lane E).

Tables

  • Figures
  • TABLE 1.

    Susceptibilities of porin-deficient K. pneumoniae strain C2 containing various plasmid-mediated β-lactamases

    Enzyme groupβ-LactamasePlasmidMIC (μg/ml)
    CefepimeCefotaximeCefoxitinCeftazidimeErtapenemImipenemMeropenem
    None0.50.5640.5110.25
    1ACC-1pSLK548128≥1,024≥2563284
    ACT-1pMG2512128≥1,02464≥1286416
    CMY-2pMG2504128≥1,02412832328
    DHA-1pMG2474256≥1,024≥256128648
    FOX-1pGL3a16≥256≥1,024≥2561286416
    FOX-3p1734232≥1,024128822
    FOX-5pMG252464≥1,024128821
    LAT-1pHP158≥256≥1,024≥256643216
    MIR-1pMG233264≥1,0243232328
    MOX-1pRMOX-1432≥1,024643288
    MOX-2pKOL864≥1,0241281622
    2bTEM-1R10.50.5640.5210.5
    TEM-2RP140.5641822
    2beTEM-3pCFF043212864128821
    TEM-4pUD163212864321624
    TEM-5pCFF141616642561641
    TEM-6pMG22616416≥2563211
    TEM-7pIF10032264641622
    TEM-8Plasmid from E. coli CF804321664≥2561620.5
    TEM-9pMG228321664≥2563240.5
    TEM-10pMG22316464≥2561620.5
    TEM-11pMG24416264641624
    TEM-12pMG22416264641620.5
    TEM-15pMG274166464≥256822
    TEM-16Plasmid from K. pneumoniae CF130416464≥256810.5
    TEM-19pMG28986432641641
    TEM-20pUD3064326416422
    TEM-21pUD3116128642561640.5
    TEM-22Plasmid from E. coli HB101 TEM-2286464≥2561620.25
    TEM-24Plasmid from K. pneumoniae CF1104163264≥25680.50.5
    TEM-25Plasmid from E. coli CF1609163264321640.25
    TEM-26pMG22532464≥256810.25
    TEM-52pMG2768326464840.5
    TEM-61pMG2908416≥2561642
    TEM-71pMG25910.564256840.125
    TEM-88pMG27283264641620.5
    2bSHV-1R10100.50.5640.520.50.5
    2beSHV-2pMG25816464≥256810.25
    SHV-3pUD18326464≥256421
    SHV-4pUD218166425640.50.25
    SHV-5pAFF2163264≥256822
    SHV-6pSLH4710.5643280.51
    SHV-12pMG2424432≥256422
    SHV-18pMG266486464812
    CTX-M-5pCLL3417a128≥256644821
    CTX-M-14pMG2673232648822
    2bHMS-1R9970.50.564120.51
    2cPSE-1pMG2170.50.5644210.5
    PSE-4pUZ8::Tn14050.50.5640.5212
    CARB-3pUZ8::Tn140810.5640.5111
    CARB-4pUZ8::Tn14130.50.25320.5112
    SAR-1pUK6570.50.5640.5210.5
    2dOXA-1RGN23840.5640.5212
    OXA-2R460.50.564163221
    OXA-3R550.250.2516210.52
    OXA-4pMG20310.5640.5110.25
    OXA-5pMG540.50.5640.5110.25
    OXA-7pMG20211321112
    OXA-10pUZ8::Tn14040.50.5640.5212
    LCR-1pUZ8::Tn14120.50.5640.520.51
    2fKPC-1pBR322-catI-blaKPC-1a12812812864≥128≥128≥128
    3VIM-2pNOR2001a464128326464128
    • ↵a Multicopy recombinant plasmid.

  • TABLE 2.

    Susceptibilities of K. pneumoniae 002(pMG251) and its derivatives to β-lactams

    StrainMIC (μg/ml)
    CefepimeCefotaximeCefoxitinCeftazidimeErtapenemImipenemMeropenem
    002(pMG251)0.125251280.520.125
    002(pMG251) Ertapenemr MIC 4a132≥1,024164161
    002(pMG251) Ertapenemr MIC 4 carrying pQE7Kb0.516≥1,0248220.5
    002(pMG251) Ertapenemr MIC 4 carrying pSHA25Kc0.125251280.2520.125
    002(pMG251) Ertapenemr MIC 128d232≥1,024≥32128≥12816
    002(pMG251) Ertapenemr MIC 128 carrying pQE7K132≥1,024≥321682
    002(pMG251) Ertapenemr MIC 128 carrying pSHA25K0.06151280.2520.125
    • ↵a Ertapenemr MIC 4, resistant derivative for which the ertapenem MIC was 4 μg/ml.

    • ↵b Codes for OmpK36.

    • ↵c Codes for OmpK37.

    • ↵d Ertapenemr MIC 128, resistant derivative for which the ertapenem MIC was 128 μg/ml.

PreviousNext
Back to top
Download PDF
Citation Tools
Role of β-Lactamases and Porins in Resistance to Ertapenem and Other β-Lactams in Klebsiella pneumoniae
George A. Jacoby, Debra M. Mills, Nancy Chow
Antimicrobial Agents and Chemotherapy Jul 2004, 48 (8) 3203-3206; DOI: 10.1128/AAC.48.8.3203-3206.2004

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Antimicrobial Agents and Chemotherapy article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Role of β-Lactamases and Porins in Resistance to Ertapenem and Other β-Lactams in Klebsiella pneumoniae
(Your Name) has forwarded a page to you from Antimicrobial Agents and Chemotherapy
(Your Name) thought you would be interested in this article in Antimicrobial Agents and Chemotherapy.
Share
Role of β-Lactamases and Porins in Resistance to Ertapenem and Other β-Lactams in Klebsiella pneumoniae
George A. Jacoby, Debra M. Mills, Nancy Chow
Antimicrobial Agents and Chemotherapy Jul 2004, 48 (8) 3203-3206; DOI: 10.1128/AAC.48.8.3203-3206.2004
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About AAC
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AACJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

Copyright © 2019 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0066-4804; Online ISSN: 1098-6596