Article Figures & Data
Figures
- FIG. 1.
Effect of the metabolites and GPG-NH2 on HIV-1 replication. Each test compound was added to HIV-1-infected H9 cells at 100 μM, and the level of production of p24 was measured. The data represent mean values and standard deviations for three to four samples.
- FIG. 2.
Production of p24 plotted against escalating drug concentration after treatment of HIV-1IIIB-infected H9 cells with G-NH2 or GPG-NH2. The IC50s were 3.2 μM for G-NH2 and 11 μM for GPG-NH2.
- FIG. 3.
Effects of treatment with GPG-NH2, G-NH2, G-OH, or cyclosporine (1 μg/ml) on the proliferation of PHA-stimulated PBMCs after 4 days. Incorporation of [3H]thymidine was measured in a β-scintillation counter and plotted (as a percentage of the thymidine incorporation for the control) against the drug concentration. The data represent mean values and standard deviations for three to four tests (n = 6).
- FIG. 4.
Morphological studies of HIV-1 virions produced from untreated or G-NH2-treated cells and analyzed with TEM. (a) Wild-type HIV-1 showing characteristic dense, cone-shaped core (left) and round, centrally placed tangential sections (middle and right); (b) HIV-1 treated with 100 μM G-NH2 showing empty, multiple core structures (right); (c) HIV-1 treated with 100 μM G-NH2 showing a double core (left and above) and distorted core structure (right); (d) HIV-1 treated with 100 μM G-NH2 showing an elongated core in an enlarged virus envelope (below), a hollow core (left above), and a double core structure (right). Bars, 100 nm.
