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Mechanisms of Resistance

In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae

Philippe Bidet, Béatrice Burghoffer, Valérie Gautier, Naïma Brahimi, Patricia Mariani-Kurkdjian, Alaa El-Ghoneimi, Edouard Bingen, Guillaume Arlet
Philippe Bidet
1Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA 3105), Université Denis Diderot-Paris VII, Service de Microbiologie
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Béatrice Burghoffer
2Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI, Paris, France
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Valérie Gautier
2Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI, Paris, France
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Naïma Brahimi
1Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA 3105), Université Denis Diderot-Paris VII, Service de Microbiologie
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Patricia Mariani-Kurkdjian
1Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA 3105), Université Denis Diderot-Paris VII, Service de Microbiologie
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Alaa El-Ghoneimi
3Service de Chirurgie viscérale, Hôpital Robert Debré
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Edouard Bingen
1Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA 3105), Université Denis Diderot-Paris VII, Service de Microbiologie
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  • For correspondence: edouard.bingen@rdb.ap-hop-paris.fr
Guillaume Arlet
2Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI, Paris, France
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DOI: 10.1128/AAC.49.8.3562-3565.2005
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    FIG. 1.

    PFGE analysis. (A) PFGE patterns of SpeI-restricted DNA from K. pneumoniae. Lane 1, isolate K1; lanes 2 and 3, isolate K2; lane 4, outbreak isolate (SLK54) producing ACC-1 β-lactamase (14); lanes 5 to 8, unrelated strains of K. pneumoniae; lane M, molecular weight marker (lambda ladder; Bio-Rad). (B) PFGE patterns of NotI-restricted DNA from E. coli. Lanes 1 to 3, unrelated strains of E. coli; lane 4, isolate E1; lane 5, isolate E2; lane M, molecular weight marker (lambda ladder; Bio-Rad).

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    FIG. 2.

    Outer membrane protein profiles of K. pneumoniae clinical isolates K1 and K2 expressing ACC-1. Outer membrane proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in 12% acrylamide. Lane 1, molecular size standard; lane 2, isolate K1 (imipenem susceptible); lane 3, isolate K2 (imipenem resistant).

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  • TABLE 1.

    MICs of β-lactam drugs and strains characteristics

    Isolate, strain or transconjugantsSpeciesOriginβ-Lactam resistance mechanismMIC (μg/ml)a
    AMXFOXCTXCAZCPMCPM (+ CLX)bIPMIPM (+ CLX)Associated resistancec
    K1 K. pneumoniae Digestive floraACC-1 + TEM-1>256432640.750.250.1250.125KTGNt + Sxt
    K2 K. pneumoniae Digestive floraACC-1 + TEM-1 + permeability alteration>25664>329616180.25KTGNt + Sxt
    E1 E. coli UTIdTEM-1>25640.0640.250.094NDe0.125ND
    E2 E. coli Digestive floraACC-1 + TEM-1>256432640.75ND0.125NDKTGNt + Sxt
    J53 E. coli Recipient strain2ND0.0320.0470.016ND0.125NDRif
    J53-K1, J53-K2, J53-E2 E. coli TransconjugantsACC-1>2564360.125ND0.125NDKTGNt + Rif
    • ↵ a MICs were determined by the E-test method (Biodisk, Solna, Sweden). Abbreviations: AMX, amoxicillin; FOX, cefoxitin; CTX, cefotaxime; CAZ, ceftazidime; CPM, cefepime; IPM, imipenem.

    • ↵ b MICs were determined on Mueller-Hinton agar containing cloxacillin (CLX) 250 μg/ml.

    • ↵ c KTGNt, resistance to kanamycin, tobramycin, gentamicin, and netilmicin; Sxt, resistance to cotrimoxazole (trimethoprim-sulfamethoxazole); Rif, resistance to rifampin.

    • ↵ d UTI, urinary tract infection.

    • ↵ e ND, not determined.

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In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae
Philippe Bidet, Béatrice Burghoffer, Valérie Gautier, Naïma Brahimi, Patricia Mariani-Kurkdjian, Alaa El-Ghoneimi, Edouard Bingen, Guillaume Arlet
Antimicrobial Agents and Chemotherapy Jul 2005, 49 (8) 3562-3565; DOI: 10.1128/AAC.49.8.3562-3565.2005

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In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae
Philippe Bidet, Béatrice Burghoffer, Valérie Gautier, Naïma Brahimi, Patricia Mariani-Kurkdjian, Alaa El-Ghoneimi, Edouard Bingen, Guillaume Arlet
Antimicrobial Agents and Chemotherapy Jul 2005, 49 (8) 3562-3565; DOI: 10.1128/AAC.49.8.3562-3565.2005
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KEYWORDS

Bacterial Proteins
Conjugation, Genetic
Escherichia coli
Klebsiella pneumoniae
plasmids
beta-lactamases

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