Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Antimicrobial Agents and Chemotherapy
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mechanisms of Resistance

qnrB, Another Plasmid-Mediated Gene for Quinolone Resistance

George A. Jacoby, Kelley E. Walsh, Debra M. Mills, Victoria J. Walker, Herin Oh, Ari Robicsek, David C. Hooper
George A. Jacoby
1Lahey Clinic, Burlington
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: george.a.jacoby@lahey.org
Kelley E. Walsh
1Lahey Clinic, Burlington
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Debra M. Mills
1Lahey Clinic, Burlington
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Victoria J. Walker
1Lahey Clinic, Burlington
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Herin Oh
2Massachusetts General Hospital, Boston, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ari Robicsek
2Massachusetts General Hospital, Boston, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David C. Hooper
2Massachusetts General Hospital, Boston, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/AAC.50.4.1178-1182.2006
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    CLUSTAL W alignment of QnrA, QnrB1, QnrB2, and QnrS.

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    Maps of cloned qnrB genes. The sizes of the cloned fragments vary, as indicated by the 1-kb reference bars. Orf1 resembles pspF, Orf2 is related to genes of unknown function in various gram-negative bacteria, and Orf3 resembles sapA.

  • FIG. 3.
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    QnrB1 protection of DNA gyrase from ciprofloxacin inhibition of supercoiling. Reaction mixtures of 30 μl were analyzed by agarose gel electrophoresis. Reaction mixtures contained 0.2 μg relaxed pBR322 DNA (lanes 1 to 14), 6.7 nM gyrase (lanes 2 to 14), 2 μg/ml ciprofloxacin (lanes 3 to 14), and QnrB-His6 fusion protein at 25 μM (lane 4), 5 μM (lane 5), 2.5 μM (lane 6), 0.5 μM (lane 7), 50 nM (lane 8), 5 nM (lane 9), 0.5 nM (lane 10), 50 pM (lane 11), 5 pM (lane 12), or 0.5 pM (lane 13).

Tables

  • Figures
  • TABLE 1.

    Properties of QnrB plasmids

    PlasmidSpecies or strain found in:Locationβ-Lactamase(s)Other resistancesaOrf1005
    pMG298 K. pneumoniae 17Southern IndiaCTX-M-15Cm Gm Km Sm Su Tc Tm Tp+
    K. pneumoniae 21Southern IndiaSHV-1 and SHV-12Tra−−
    pMG299 K. pneumoniae 24Southern IndiaCTX-M-15Ak Gm Km Sm Su Tm Tp+
    pMG300 K. pneumoniae 25Southern IndiaSHV-12Gm Km Sm Tm+
    pMG301 C. koseri Long Beach, CASHV-12Gm Sm Su Tp−
    pMG302 E. cloacae Philadelphia, PASHV-12Cm Gm Km Sm Su Tm Tp−
    pMG303 E. cloacae St. Louis, MOSHV-12Cm Km Sm Su Tc Tp−
    pMG304 E. coli St. Louis, MOSHV-12Cm Km Sm Su−
    • ↵ a Resistance abbreviations: Ak, amikacin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Su, sulfonamide; Tc, tetracycline; Tm, tobramycin; Tp, trimethoprim.

  • TABLE 2.

    Susceptibility of E. coli J53 transconjugants to various quinolones

    Plasmid in E. coli J53Qnr proteinMIC (μg/ml)
    Nalidixic acidCiprofloxacinGatifloxacinLevofloxacinMoxifloxacin
    R−40.0150.030.030.06
    pMG252QnrA320.50.511
    pHSH4-3QnrA160.250.50.50.5
    pMG298QnrB116110.52
    pMG299QnrB1160.250.50.51
    pMG300QnrB1160.250.50.251
PreviousNext
Back to top
Download PDF
Citation Tools
qnrB, Another Plasmid-Mediated Gene for Quinolone Resistance
George A. Jacoby, Kelley E. Walsh, Debra M. Mills, Victoria J. Walker, Herin Oh, Ari Robicsek, David C. Hooper
Antimicrobial Agents and Chemotherapy Mar 2006, 50 (4) 1178-1182; DOI: 10.1128/AAC.50.4.1178-1182.2006

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Antimicrobial Agents and Chemotherapy article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
qnrB, Another Plasmid-Mediated Gene for Quinolone Resistance
(Your Name) has forwarded a page to you from Antimicrobial Agents and Chemotherapy
(Your Name) thought you would be interested in this article in Antimicrobial Agents and Chemotherapy.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
qnrB, Another Plasmid-Mediated Gene for Quinolone Resistance
George A. Jacoby, Kelley E. Walsh, Debra M. Mills, Victoria J. Walker, Herin Oh, Ari Robicsek, David C. Hooper
Antimicrobial Agents and Chemotherapy Mar 2006, 50 (4) 1178-1182; DOI: 10.1128/AAC.50.4.1178-1182.2006
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Bacterial Proteins
plasmids

Related Articles

Cited By...

About

  • About AAC
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • AAC Podcast
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AACJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0066-4804; Online ISSN: 1098-6596