Article Figures & Data
Figures
- FIG. 1.
Localization of the blaKPC-2 gene. (A) Electrophoretic profiles of plasmids digested with the indicated enzyme. (B) Hybridization with a blaKPC-2-specific probe. Lanes: 1 to 3, P. aeruginosa PA2404, PA4012, and PA4036, respectively; 4, C. freundii CF6010; 5, KPN633 (positive control) (22); M, digoxygenin-labeled marker; T and S, TEM-10- and SHV-12-containing plasmids, respectively (negative controls).
- FIG. 2.
Localization of the blaKPC gene in I-CeuI-generated, pulsed-field gel electrophoresis-separated chromosome fragments of P. aeruginosa and C. freundii. (A) I-CeuI fragment restriction pattern. (B) Hybridization with a probe specific to 16S rRNA genes. (C) Hybridization with a probe specific to blaKPC. Lanes: 1, P. aeruginosa ATCC 27853; 2, P. aeruginosa PA4036; 3, C. freundii CH32 (imipenem-sensitive isolate); 4, C. freundii CF6010; M, lambda DNA ladder standard with sizes (in kilobases) indicated on the left.
Tables
- TABLE 1.
Antimicrobial susceptibility patterns of P. aeruginosa and C. freundii KPC-possessing clinical isolates
Antimicrobial agent(s) MIC (μg/ml) P. aeruginosa PA2404 P. aeruginosa PA4012 P. aeruginosa PA4036 C. freundii CF6010 Imipenem 256 >256 >256 8 Imipenem-CLAa 256 >256 >256 4 Meropenem >256 >256 >256 8 Meropenem-CLA >256 >256 >256 2 Ertapenem >256 256 >256 16 Ampicillin >256 >256 >256 >256 Ampicillin-SULb >256 >256 >256 >256 Piperacillin >256 >256 >256 >256 Piperacillin-TZBc >256 >256 >256 >256 Ceftazidime 256 256 256 32 Cefotaxime >256 >256 >256 32 Ceftriaxone >256 >256 >256 256 Cefepime >256 >256 >256 8 Aztreonam >256 >256 >256 128 Aztreonam-CLA >256 >256 >256 128 Amikacin 16 16 16 2 Gentamicin >128 >128 >128 >128 Tobramycin >128 >128 >128 64 Tigecyclined 16 16 16 0.5 Colistin 1 2 1 0.5
