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Mechanisms of Resistance

First Identification of Pseudomonas aeruginosa Isolates Producing a KPC-Type Carbapenem-Hydrolyzing β-Lactamase

Maria Virginia Villegas, Karen Lolans, Adriana Correa, Juan Nicolas Kattan, Jaime A. Lopez, John P. Quinn
and and the Colombian Nosocomial Resistance Study Group
Maria Virginia Villegas
CIDEIM (International Center for Medical Research and Training), Cali, Colombia
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Karen Lolans
John Stroger Hospital, Chicago, IllinoisChicago Infectious Disease Research Institute, Chicago, Illinois
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Adriana Correa
CIDEIM (International Center for Medical Research and Training), Cali, Colombia
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Juan Nicolas Kattan
CIDEIM (International Center for Medical Research and Training), Cali, Colombia
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Jaime A. Lopez
Hospital Pablo Tobon Uribe, Medellin, Colombia
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John P. Quinn
John Stroger Hospital, Chicago, IllinoisChicago Infectious Disease Research Institute, Chicago, IllinoisRush University Medical Center, Chicago, Illinois
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  • For correspondence: ESBLman@yahoo.com
DOI: 10.1128/AAC.01405-06
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  • FIG. 1.
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    FIG. 1.

    Localization of the blaKPC-2 gene. (A) Electrophoretic profiles of plasmids digested with the indicated enzyme. (B) Hybridization with a blaKPC-2-specific probe. Lanes: 1 to 3, P. aeruginosa PA2404, PA4012, and PA4036, respectively; 4, C. freundii CF6010; 5, KPN633 (positive control) (22); M, digoxygenin-labeled marker; T and S, TEM-10- and SHV-12-containing plasmids, respectively (negative controls).

  • FIG. 2.
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    FIG. 2.

    Localization of the blaKPC gene in I-CeuI-generated, pulsed-field gel electrophoresis-separated chromosome fragments of P. aeruginosa and C. freundii. (A) I-CeuI fragment restriction pattern. (B) Hybridization with a probe specific to 16S rRNA genes. (C) Hybridization with a probe specific to blaKPC. Lanes: 1, P. aeruginosa ATCC 27853; 2, P. aeruginosa PA4036; 3, C. freundii CH32 (imipenem-sensitive isolate); 4, C. freundii CF6010; M, lambda DNA ladder standard with sizes (in kilobases) indicated on the left.

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  • TABLE 1.

    Antimicrobial susceptibility patterns of P. aeruginosa and C. freundii KPC-possessing clinical isolates

    Antimicrobial agent(s)MIC (μg/ml)
    P. aeruginosa PA2404P. aeruginosa PA4012P. aeruginosa PA4036C. freundii CF6010
    Imipenem256>256>2568
    Imipenem-CLAa256>256>2564
    Meropenem>256>256>2568
    Meropenem-CLA>256>256>2562
    Ertapenem>256256>25616
    Ampicillin>256>256>256>256
    Ampicillin-SULb>256>256>256>256
    Piperacillin>256>256>256>256
    Piperacillin-TZBc>256>256>256>256
    Ceftazidime25625625632
    Cefotaxime>256>256>25632
    Ceftriaxone>256>256>256256
    Cefepime>256>256>2568
    Aztreonam>256>256>256128
    Aztreonam-CLA>256>256>256128
    Amikacin1616162
    Gentamicin>128>128>128>128
    Tobramycin>128>128>12864
    Tigecyclined1616160.5
    Colistin1210.5
    • ↵a CLA, clavulanic acid; inhibitor tested at a fixed concentration of 2 μg/ml.

    • ↵b SUL, sulbactam. Penicillin/inhibitor ratio, 2:1.

    • ↵c TZB, tazobactam; inhibitor tested at a fixed concentration of 4 μg/ml.

    • ↵d Tested in CA-MHB medium <12 h old.

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First Identification of Pseudomonas aeruginosa Isolates Producing a KPC-Type Carbapenem-Hydrolyzing β-Lactamase
Maria Virginia Villegas, Karen Lolans, Adriana Correa, Juan Nicolas Kattan, Jaime A. Lopez, John P. Quinn and the Colombian Nosocomial Resistance Study Group
Antimicrobial Agents and Chemotherapy Mar 2007, 51 (4) 1553-1555; DOI: 10.1128/AAC.01405-06

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First Identification of Pseudomonas aeruginosa Isolates Producing a KPC-Type Carbapenem-Hydrolyzing β-Lactamase
Maria Virginia Villegas, Karen Lolans, Adriana Correa, Juan Nicolas Kattan, Jaime A. Lopez, John P. Quinn and the Colombian Nosocomial Resistance Study Group
Antimicrobial Agents and Chemotherapy Mar 2007, 51 (4) 1553-1555; DOI: 10.1128/AAC.01405-06
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