Article Figures & Data
Figures
- FIG. 1.
Restriction analysis (PstI) (upper panel) and Southern blot hybridization with a blaTEM-52 probe (lower panel) of plasmid DNAs isolated from E. coli transconjugants. Lane 1, E. coli transconjugant 988SA01TC1; lane 2, E. coli transconjugant 2004/10101TC1; lane 3, E. coli transconjugant 2004/10256TC1; lane 4, E. coli transconjugant 05-00001TC1; lane 5, E. coli transconjugant 05-00590TC1; lane 6, E. coli transconjugant 05-00838TC1; lane 7, E. coli transconjugant 1043SA04TC1; lane 8, E. coli transconjugant 777SA01TC1; lane 9, E. coli transconjugant 260SA04TC1; lane 10, E. coli transconjugant 833SA04TC1; lane 11, E. coli transconjugant 153SA02TC1; lane 12, E. coli transconjugant 04-3486TC1; lane 13, E. coli transconjugant 977SA01TC1; lane 14, E. coli transconjugant pPAN-1 (the latter strain served as control and was previously published (14); lane M, markers (Smartladder; Eurogentec, Seraing, Belgium).
- FIG. 2.
Genetic organization of the blaTEM-52 carrying transposon on a conjugative IncI1 plasmid from serovar Typhimurium strain 04-3486. The position and orientation of the genes are indicated by arrows. Gray arrows correspond to plasmid genes flanking the transposon. IRL and IRR correspond, respectively, to left and right terminal inverted repeats of Tn3 and are indicated as black arrowheads. The sequences of IRL and IRR are also indicated. The 5-bp direct repeat at the integration site of Tn3 downstream IRR is indicated in boldface letters. The black box between tnpA and tnpR indicates the resolution site of Tn3. The nucleotide sequence of TnblaTEM-52 has been deposited under GenBank accession number EF141186. A distance scale in kilobase pairs is given above the map.
Tables
- TABLE 1.
Characteristics of the Salmonella strains and their transconjugants producing TEM-52 used in this study
Straina Serovar Geographic origin Animal or human origin Yr of isolation Antibiotic resistance profileb MIC (μg/ml)c SGI1 variantd Xnl Cro 777SA01 Agona Belgium Poultry 2001 Ap(Caz)CfCm(Cro)FfSmSpSuTcTm(Xnl) 16 16 SGI1-A 777SA01TC1 Ap(Caz)Cf(Cro)(Xnl) 4 2 260SA04 Agona Belgium Poultry 2004 ApCazCfCmCroFfSmSuTcTmXnl 64 256 — 260SA04TC1 Ap(Caz)Cf(Cro)(Xnl) 2 1 833SA04 Agona Belgium Poultry 2004 ApCazCfCroXnl 32 64 — 833SA04TC1 Ap(Caz)Cf(Cro)(Xnl) 2 1 977SA01 Derby Belgium Poultry 2001 ApCazCfCroXnl 32 16 — 977SA01TC1 Ap(Caz)Cf(Cro)(Xnl) 4 4 988SA01 Infantis Belgium Poultry 2001 ApCazCfCroXnl 32 32 — 988SA01TC1 Ap(Caz)Cf(Cro)(Xnl) 2 1 2004/10101 Infantis Belgium Poultry 2004 ApCazCfCroXnl 32 128 — 2004/10101TC1 Ap(Caz)Cf(Cro)(Xnl) 2 4 2004/10256 Infantis Belgium Poultry 2004 ApCazCfCroXnl 32 64 — 2004/10256TC1 Ap(Caz)Cf(Cro)(Xnl) 2 2 05-00001 Infantis Belgium Human 2005 ApCazCfCroXnl 32 64 — 05-00001TC1 Ap(Caz)Cf(Cro)(Xnl) 4 4 05-00590 Infantis Belgium Human 2005 ApCazCfCroXnl 32 64 — 05-00590TC1 Ap(Caz)Cf(Cro)(Xnl) 2 4 05-00838 Infantis Belgium Human 2005 ApCazCfCroXnl 16 64 — 05-00838TC1 Ap(Caz)Cf(Cro)(Xnl) 4 4 1043SA04 Paratyphi B Belgium Poultry 2004 ApCazCfCroSmSpSuTmXnl 32 64 — 1043SA04TC1 Ap(Caz)Cf(Cro)(Xnl) 4 4 153SA02 Typhimurium Belgium Poultry 2002 ApCazCfCroSmSpSuTmXnl 32 32 — 153SA02TC1 Ap(Caz)Cf(Cro)(Xnl) 2 1 04-3486 Typhimurium France Human 2004 ApCazCfCm(Cro)FfSmSpSuTc(Xnl) 16 32 SGI1 04-3486TC1 Ap(Caz)Cf(Cro)(Xnl) 2 2 ↵ a Strains labeled with TC1 are E. coli transconjugant strains.
↵ b Antibiotics: Ap, ampicillin; Caz, ceftazidime; Cf, cefalothin; Cm, chloramphenicol; Cro, ceftriaxone; Ff, florfenicol; Sm, streptomycin; Sp, spectinomycin; Su, sulfonamide; Tc, tetracycline; Tm, trimethoprim; Xnl, ceftiofur. Parentheses indicate intermediate resistance according to the breakpoints of the CA-SFM (Comité de l'Antibiogramme de la Société Française de Microbiologie) for Enterobacteriaceae (i.e., susceptible, >20 mm; resistant, <15 mm).
↵ c Cro, ceftriaxone; Xnl, ceftiofur.
↵ d —, negative for SGI1.
