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Mechanisms of Resistance

Dissemination of an Extended-Spectrum-β-Lactamase blaTEM-52 Gene-Carrying IncI1 Plasmid in Various Salmonella enterica Serovars Isolated from Poultry and Humans in Belgium and France between 2001 and 2005

Axel Cloeckaert, Karine Praud, Benoît Doublet, Alessia Bertini, Alessandra Carattoli, Patrick Butaye, Hein Imberechts, Sophie Bertrand, Jean-Marc Collard, Guillaume Arlet, François-Xavier Weill
Axel Cloeckaert
1INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly F-37380, France
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  • For correspondence: cloeckae@tours.inra.fr
Karine Praud
1INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly F-37380, France
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Benoît Doublet
1INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly F-37380, France
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Alessia Bertini
2Department of Infectious, Parasitic, Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
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Alessandra Carattoli
2Department of Infectious, Parasitic, Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
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Patrick Butaye
3Department of Bacteriology and Immunology, CODA-CERVA-VAR, Groeselenberg 99, 1180 Brussels, Belgium
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Hein Imberechts
3Department of Bacteriology and Immunology, CODA-CERVA-VAR, Groeselenberg 99, 1180 Brussels, Belgium
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Sophie Bertrand
4National Reference Centre for Salmonella and Shigella, Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman St., B-1050 Brussels, Belgium
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Jean-Marc Collard
4National Reference Centre for Salmonella and Shigella, Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman St., B-1050 Brussels, Belgium
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Guillaume Arlet
5Département de Bactériologie, UPRES EA2392, Faculté de Médecine Pierre et Marie Curie, 27 Rue de Chaligny, 75012 Paris, France
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François-Xavier Weill
6Centre National de Référence des Salmonella, Unité de Biodiversité des Bactéries Pathogènes Emergentes, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
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DOI: 10.1128/AAC.01514-06
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  • FIG. 1.
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    FIG. 1.

    Restriction analysis (PstI) (upper panel) and Southern blot hybridization with a blaTEM-52 probe (lower panel) of plasmid DNAs isolated from E. coli transconjugants. Lane 1, E. coli transconjugant 988SA01TC1; lane 2, E. coli transconjugant 2004/10101TC1; lane 3, E. coli transconjugant 2004/10256TC1; lane 4, E. coli transconjugant 05-00001TC1; lane 5, E. coli transconjugant 05-00590TC1; lane 6, E. coli transconjugant 05-00838TC1; lane 7, E. coli transconjugant 1043SA04TC1; lane 8, E. coli transconjugant 777SA01TC1; lane 9, E. coli transconjugant 260SA04TC1; lane 10, E. coli transconjugant 833SA04TC1; lane 11, E. coli transconjugant 153SA02TC1; lane 12, E. coli transconjugant 04-3486TC1; lane 13, E. coli transconjugant 977SA01TC1; lane 14, E. coli transconjugant pPAN-1 (the latter strain served as control and was previously published (14); lane M, markers (Smartladder; Eurogentec, Seraing, Belgium).

  • FIG. 2.
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    FIG. 2.

    Genetic organization of the blaTEM-52 carrying transposon on a conjugative IncI1 plasmid from serovar Typhimurium strain 04-3486. The position and orientation of the genes are indicated by arrows. Gray arrows correspond to plasmid genes flanking the transposon. IRL and IRR correspond, respectively, to left and right terminal inverted repeats of Tn3 and are indicated as black arrowheads. The sequences of IRL and IRR are also indicated. The 5-bp direct repeat at the integration site of Tn3 downstream IRR is indicated in boldface letters. The black box between tnpA and tnpR indicates the resolution site of Tn3. The nucleotide sequence of TnblaTEM-52 has been deposited under GenBank accession number EF141186. A distance scale in kilobase pairs is given above the map.

Tables

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  • TABLE 1.

    Characteristics of the Salmonella strains and their transconjugants producing TEM-52 used in this study

    StrainaSerovarGeographic originAnimal or human originYr of isolationAntibiotic resistance profilebMIC (μg/ml)cSGI1 variantd
    XnlCro
    777SA01AgonaBelgiumPoultry2001Ap(Caz)CfCm(Cro)FfSmSpSuTcTm(Xnl)1616SGI1-A
    777SA01TC1Ap(Caz)Cf(Cro)(Xnl)42
    260SA04AgonaBelgiumPoultry2004ApCazCfCmCroFfSmSuTcTmXnl64256—
    260SA04TC1Ap(Caz)Cf(Cro)(Xnl)21
    833SA04AgonaBelgiumPoultry2004ApCazCfCroXnl3264—
    833SA04TC1Ap(Caz)Cf(Cro)(Xnl)21
    977SA01DerbyBelgiumPoultry2001ApCazCfCroXnl3216—
    977SA01TC1Ap(Caz)Cf(Cro)(Xnl)44
    988SA01InfantisBelgiumPoultry2001ApCazCfCroXnl3232—
    988SA01TC1Ap(Caz)Cf(Cro)(Xnl)21
    2004/10101InfantisBelgiumPoultry2004ApCazCfCroXnl32128—
    2004/10101TC1Ap(Caz)Cf(Cro)(Xnl)24
    2004/10256InfantisBelgiumPoultry2004ApCazCfCroXnl3264—
    2004/10256TC1Ap(Caz)Cf(Cro)(Xnl)22
    05-00001InfantisBelgiumHuman2005ApCazCfCroXnl3264—
    05-00001TC1Ap(Caz)Cf(Cro)(Xnl)44
    05-00590InfantisBelgiumHuman2005ApCazCfCroXnl3264—
    05-00590TC1Ap(Caz)Cf(Cro)(Xnl)24
    05-00838InfantisBelgiumHuman2005ApCazCfCroXnl1664—
    05-00838TC1Ap(Caz)Cf(Cro)(Xnl)44
    1043SA04Paratyphi BBelgiumPoultry2004ApCazCfCroSmSpSuTmXnl3264—
    1043SA04TC1Ap(Caz)Cf(Cro)(Xnl)44
    153SA02TyphimuriumBelgiumPoultry2002ApCazCfCroSmSpSuTmXnl3232—
    153SA02TC1Ap(Caz)Cf(Cro)(Xnl)21
    04-3486TyphimuriumFranceHuman2004ApCazCfCm(Cro)FfSmSpSuTc(Xnl)1632SGI1
    04-3486TC1Ap(Caz)Cf(Cro)(Xnl)22
    • ↵ a Strains labeled with TC1 are E. coli transconjugant strains.

    • ↵ b Antibiotics: Ap, ampicillin; Caz, ceftazidime; Cf, cefalothin; Cm, chloramphenicol; Cro, ceftriaxone; Ff, florfenicol; Sm, streptomycin; Sp, spectinomycin; Su, sulfonamide; Tc, tetracycline; Tm, trimethoprim; Xnl, ceftiofur. Parentheses indicate intermediate resistance according to the breakpoints of the CA-SFM (Comité de l'Antibiogramme de la Société Française de Microbiologie) for Enterobacteriaceae (i.e., susceptible, >20 mm; resistant, <15 mm).

    • ↵ c Cro, ceftriaxone; Xnl, ceftiofur.

    • ↵ d —, negative for SGI1.

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Dissemination of an Extended-Spectrum-β-Lactamase blaTEM-52 Gene-Carrying IncI1 Plasmid in Various Salmonella enterica Serovars Isolated from Poultry and Humans in Belgium and France between 2001 and 2005
Axel Cloeckaert, Karine Praud, Benoît Doublet, Alessia Bertini, Alessandra Carattoli, Patrick Butaye, Hein Imberechts, Sophie Bertrand, Jean-Marc Collard, Guillaume Arlet, François-Xavier Weill
Antimicrobial Agents and Chemotherapy Apr 2007, 51 (5) 1872-1875; DOI: 10.1128/AAC.01514-06

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Dissemination of an Extended-Spectrum-β-Lactamase blaTEM-52 Gene-Carrying IncI1 Plasmid in Various Salmonella enterica Serovars Isolated from Poultry and Humans in Belgium and France between 2001 and 2005
Axel Cloeckaert, Karine Praud, Benoît Doublet, Alessia Bertini, Alessandra Carattoli, Patrick Butaye, Hein Imberechts, Sophie Bertrand, Jean-Marc Collard, Guillaume Arlet, François-Xavier Weill
Antimicrobial Agents and Chemotherapy Apr 2007, 51 (5) 1872-1875; DOI: 10.1128/AAC.01514-06
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KEYWORDS

plasmids
poultry
Salmonella enterica
beta-lactamases

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