Phage Therapy of Pseudomonas aeruginosa Infection in a Mouse Burn Wound Model

DOI: 10.1128/AAC.01028-06

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FIG. 1.
Pharmacokinetics of the phages in noninfected mice. A cocktail containing ∼3 × 10⁸ phage was injected i.p., i.m., or s.c. into uninjured, uninfected mice. Groups of mice (n = 3) were killed at 0.5 h hpi, 12 hpi, 24 hpi, 36 hpi, or 48 hpi. Tissues were removed and the numbers of PFU were determined by serial dilution and plating on PAO1^Rif. (A) PFU/gram liver; (B) PFU/gram spleen; (C) PFU/ml blood. Values are means ± standard errors of the means.

TABLE 1.

Protection studies: efficacy of phage therapy on P. aeruginosa infection of burn wounds

Treatment	No. of survivors/total no. of mice (% survival)^a
	Expt 1		Expt 2		Expt 3		Combined expt^b
	48 hpi	72 hpi	48 hpi	72 hpi	48 hpi	72 hpi	48 hpi	72 hpi
PAO1 only	0/6 (0)	0/6 (0)	1/6 (16.7)	0/6 (0)	2/6 (33.3)	1/6 (16.7)	3/18 (17)	1/18 (6)
Phage by i.p. route only	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	18/18 (100)	18/18 (100)
Phage by i.m. route only	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	18/18 (100)	18/18 (100)
Phage by s.c. route only	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	6/6 (100)	18/18 (100)	18/18 (100)
Phage by i.p. route + PAO1 infection	6/6 (100)	5/6 (83.3)	6/6 (100)	6/6 (100)	5/5 (100)	4/5 (80)	17/17 (100)^c	15/17 (88)^e
Phage by i.m. route + PAO1 infection	3/6 (50)	2/6 (33.3)	3/6 (50)	0/6 (0)	4/6 (66.7)	3/6 (50)	10/18 (56)^d	5/18 (28)
Phage by s.c. route + PAO1 infection	2/6 (33.3)	2/6 (33.3)	3/6 (50)	0/6 (0)	5/6 (83.3)	2/6 (33.3)	10/18 (56)^d	4/18 (22)

↵a The cumulative survival was determined at 48 h and 72 hpi.
↵b The average percent survival of animals in three experiments determined at 48 and 72 hpi.
↵c The rate of survival for animals receiving the phage cocktail i.p. was significantly greater (P < 0.0001) than that for untreated infected control animals at the same time period.
↵d The rate of survival for animals receiving the phage cocktail i.p. was significantly greater (P < 0.01) than that for untreated infected control animals at the same time period.
↵e The rate of survival for animals receiving the phage cocktail i.p. was significantly greater (P < 0.0026) than that for untreated infected control animals at the same time period.