Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Antimicrobial Agents and Chemotherapy
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Letter to the Editor

Detection of Gene Cassettes in Tn402-Like Class 1 Integrons

Virginia Post, Gavin D. Recchia, Ruth M. Hall
Virginia Post
School of Molecular and Microbial Biosciences The University of Sydney, 2006 New South Wales, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gavin D. Recchia
Davies Collison Cave 255 Elizabeth Street Sydney, 2000, New South Wales, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruth M. Hall
School of Molecular and Microbial Biosciences The University of Sydney, 2006 New South Wales, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: ruth.hall@mmb.usyd.edu.au
DOI: 10.1128/AAC.00220-07
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Context of gene cassettes in class 1 integrons. (A) Types of class 1 integron structure. Tall vertical bars represent the inverted repeats, and the various backbone segments, 5′-CS, 3′-CS, and the tni module, are represented by lines of different thicknesses. The short vertical bar represents the res site. Open vertical bars represent the attI site of class 1 integrons. Insertion sequences (IS) are shown as open boxes with the IS number above. Genes are indicated by arrows with gene names below. Positions of the primers used here are marked by flags with the primer name adjacent. L1 (5′-GGCATCCAAGCAGCAAGC-3′) and R1 (5′-AAGCAGACTTGACCTGAT-3′) (corresponding to primers 1 and 2 in reference 8) are representative of primer pairs used to amplify cassette arrays. (B) Cassettes in Tn402 and rearranged derivatives. The SphI-BamHI fragment of Tn402 present in pRMH532 is shown together with the cassette arrays present in the various derivatives recovered in this study. Cassettes are shown as open boxes with a vertical bar (representing the 59-be) at one end. (C) PCR amplification of gene cassettes using primer L1 with primer RH506. Lanes: 1, 1-kb markers; 2, pRMH532; 3, pRMH545; 4, pRMH542; 5, pRMH544; 6, pRMH543; 7, 100-bp markers. Predicted sizes of PCR products are 1,524 bp for pRMH532 and 924, 801, 617, and 534 bp for pRMH545, pRMH542, pRMH544, and pRMH543, respectively.

Tables

  • Figures
  • TABLE 1.

    Cassette arrays flanked by the 5′-CS and tni modules

    OrganismGene cassettesGenBank accession no.Reference
    Klebsiella aerogenes dfrB3-orfD-qacE X72585 15
    Klebsiella pneumoniae Unknown U95134
    Pseudomonas aeruginosa bla VIM2-oxa2-aacA4-aadB-qacG AY507153
    Pseudomonas aeruginosa aacA7-blaVIM2-dfrB5-aacC-A5 AY943084 DQ522233 11
    Uncultured oxa2-aacA4-aadA1 AJ744860 17
    Pseudomonas aeruginosa bla VIM2-aacA4 AM180753 6
    Pseudomonas aeruginosa aacA7-blaVIM-2-dfrB5-aac6-II (ISPa21-like) AM296017
PreviousNext
Back to top
Download PDF
Citation Tools
Detection of Gene Cassettes in Tn402-Like Class 1 Integrons
Virginia Post, Gavin D. Recchia, Ruth M. Hall
Antimicrobial Agents and Chemotherapy Aug 2007, 51 (9) 3467-3468; DOI: 10.1128/AAC.00220-07

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Antimicrobial Agents and Chemotherapy article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Detection of Gene Cassettes in Tn402-Like Class 1 Integrons
(Your Name) has forwarded a page to you from Antimicrobial Agents and Chemotherapy
(Your Name) thought you would be interested in this article in Antimicrobial Agents and Chemotherapy.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Detection of Gene Cassettes in Tn402-Like Class 1 Integrons
Virginia Post, Gavin D. Recchia, Ruth M. Hall
Antimicrobial Agents and Chemotherapy Aug 2007, 51 (9) 3467-3468; DOI: 10.1128/AAC.00220-07
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Genes, Bacterial
integrons

Related Articles

Cited By...

About

  • About AAC
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • AAC Podcast
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AACJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0066-4804; Online ISSN: 1098-6596