Article Figures & Data
Figures
- FIG. 1.
Antimicrobial susceptibility testing and antimicrobial resistance gene detection results for the Salmonella serovar Newport isolates characterized in the study. The isolates are arranged by a PFGE profile similarity dendrogram (calculated in reference 19) and are described by culture number (Key), animal of origin (Species), state of origin (State), the sizes and resistance gene inserts in the class 1 integrons (Integron), and the sizes of plasmids isolated from each strain (Plasmids [kb]). For the susceptibility testing results (Resistance), a black box indicates resistance, a light gray box susceptibility, and a darker gray box reduced susceptibility to ceftriaxone. For resistance gene detection (ResGen), a black box indicates that the gene was detected by PCR, and a light gray box indicates the absence of the gene.
Tables
- TABLE 1.
PCR primers and gene targets for antimicrobial resistance
Gene Primera Nucleotide sequence (5′ to 3′) Annealing temp (°C)b Resistance mechanism Resistancec Reference or source aadA1 F TATCAGAGGTAGTTGGCGTCAT 54 Aminoglycoside adenyltransferase STR 13 R GTTCCATAGCGTTAAGGTTTCATT aadA2 F TGTTGGTTACTGTGGCCGTA 62 Aminoglycoside adenyltransferase STR 13 R GATCTCGCCTTTCACAAAGC aadB F GAGCGAAATCTGCCGCTCTGG 61 Aminoglycoside adenyltransferase GEN 13 R CTGTTACAACGGACTGGCCGC aacC F GGCGCGATCAACGAATTTATCCGA 58 Aminoglycoside acetyltransferase GEN This study R CCATTCGATGCCGAAGGAAACGAT aphAI-IAB F AAACGTCTTGCTCGAGGC 55 Aminoglycoside phosphoryltranferase KAN 13 R CAAACCGTTATTCATTCGTGA bla CMY F GACAGCCTCTCTTTCTCCACA 50 β-Lactamase AMP 13 R TGGAACGAAGGCTACGTA bla TEM F CATTTCCGTGTCGCCCTTAT 55 β-Lactamase AMP 13 R TCCATAGTTGCCTGACTCCC cat1 F CCTATAACCAGACCGTTCAG 56 Chlorampenicol acetyltransferase CHL 13 R TCACAGACGGCATGATGAAC cat2 F CCGGATTGACCTGAATACCT 56 Chlorampenicol acetyltransferase CHL 13 R TCACATACTGCATGATGAAC dfrI F GTGAAACTATCACTAATGGTAGCT 54 Dihydrofloate reductase inhibitor TMP 13 R ACCCTTTTGCCAGATTTGGTAACT floR F AACCCGCCCTCTGGATCAAGTCAA 60 Efflux CHL 13 R CAAATCACGGGCCACGCTGTATC strA F AGCAGAGCGCGCCTTCGCTC 59 Aminoglycoside phosphoryltranferase STR 13 R CCAAAGCCCACTTCACCGAC strB F ATCGTCAAGGGATTGAAACC 49 Aminoglycoside phosphoryltranferase STR 3 R GGATCGTAGAACATATTGGC sul1 F TCACCGAGGACTCCTTCTTC 60 Dihydropteroate synthase inhibitor SUL 13 R AATATCGGGATAGAGCGCAG sul2 F CGGTCCGGCATCCAGCAATCC 64 Dihydropteroate synthase inhibitor SUL 13 R CGAGAGCCACGACCGCGCC sul3 F GAGCAAGATTTTTGGAATCG 51 Dihydropteroate synthase inhibitor SUL 3 R CATCTGCAGCTAACCTAGGGCTTGGA tetA F GCTACATCCTGCTTGCCTTC 55 Efflux TET 8 R CATAGATCGCCGTGAAGAGG tetB F TTGGTTAGGGGCAAGTTTTG 53 Efflux TET 8 R GTAATGGGCCAATAACACCG tetC F CTTGAGAGCCTTCAACCCAG 56 Efflux TET 8 R ATGGTCGTCATCTACCTGCC intA F GGCATCCAAGCAGCAAGC 50 Class 1 integron 13 R AAGCAGACTTGACCTGAT intB F ATCGCAATAGTTGGCGAGT 53 qacEΔ-sul1 of class 1 integron 13 R GCAAGGCGGAAACCCGCGCC
