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LETTERS TO THE EDITOR

High Prevalence of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Bamako, Mali

Véronique Duval, Ibrahim Maiga, Aminata Maiga, Thomas Guillard, Lucien Brasme, Dominique Forte, Janick Madoux, Véronique Vernet-Garnier, Christophe De Champs
Véronique Duval
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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Ibrahim Maiga
Laboratoire de Microbiologie Hôpital du Point G, BP 333 Bamako, Mali
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Aminata Maiga
Laboratoire de Microbiologie Hôpital du Point G, BP 333 Bamako, Mali
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Thomas Guillard
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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Lucien Brasme
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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Dominique Forte
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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Janick Madoux
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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Véronique Vernet-Garnier
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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Christophe De Champs
Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière CHU Reims UFR Médecine Reims Champagne-Ardenne 51092 Reims, France
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  • For correspondence: cdechamps@chu-reims.fr
DOI: 10.1128/AAC.00675-09
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In 2004, Weil et al. reported the presence of CTX-M-15 in Salmonella enterica (10), and recently Ruppé et al. reported the carriage of CTX-M-15-producing isolates in children in a remote village in Senegal (8). However, since the report of SHV-type enzymes in Nigeria (1), no survey has been performed on extended-spectrum β-lactamase (ESBL) producers in West African hospitals (4, 7). Between May 2004 and April 2006, in Hôpital du Point G, Bamako, Mali, of 1,193 nonrepetitive isolates of Enterobacteriaceae identified by the API 20E system (bioMérieux, Marcy l'Étoile, France), 256 produced an ESBL detected by the double-disk synergy test (9) (156/747 Escherichia coli, 82/217 Klebsiella pneumoniae, 15/81 Enterobacter cloacae, 2/12 Morganella morganii, and 1/36 Proteus mirabilis isolates). Enzymes were identified for 109 E. coli and 63 K. pneumoniae isolates randomly selected every 3 months and for all E. cloacae, M. morganii, and P. mirabilis isolates. These strains were isolated from patients hospitalized in the medical (n = 127 [66.3%]) and surgical (n = 27 [14.0%]) wards and intensive care units (n = 2 [1.2%]) and emergency patients (n = 4 [2.3%]) and outpatients (n = 30 [15.7%]) and from urine (n = 142 [74.7%]), blood (n = 12 [6.3%]), pus (n = 20 [10.4%]), and other sites (n = 16 [8.3%]) (stool, catheter, and pleural and peritoneal fluid). Antimicrobial susceptibility was determined by disk diffusion according to the recommendations of the Antibiogram Committee of the French Society for Microbiology (http://www.sfm.asso.fr/ ) (Table 1). Isoelectric focusing evidenced only one pI corresponding to a TEM-type enzyme, pI 5.4, in 121/190 isolates. Since this pI was considered as TEM-1 in most cases, we did not screen other TEM-type ESBLs by PCR. All 190 isolates were positive for blaSHV- or blaCTX-M-related genes, as assessed by PCR using previously described specific primers (2). After direct sequencing of PCR products (2), CTX-M-15 (n = 157), CTX-M-14 (n = 17), SHV-12 (n = 17), and SHV-27 (n = 1) were identified. Two isolates produced two ESBLs, CTX-M-15 and SHV-12 or SHV-27 (Table 1). Except in three isolates of E. coli, all SHV-type enzymes were produced by E. cloacae or K. pneumoniae, confirming the role of E. coli CTX-M-15 in the increase in ESBL incidence. To determine whether the ESBL frequency was due to the spread of some predominant strains, randomly amplified polymorphic DNA (RAPD) analysis was performed (2). A great diversity of RAPD types were observed (Table 1), and no isolate was the same as E. coli O25:H4-ST 131 (3, 5). In E. cloacae species, all SHV-12-producing isolates had similar RAPD patterns, in contrast to CTX-M-15-producing isolates, which were all different.

This study confirms that the worldwide diffusion of the CTX-M-15 enzyme has also reached West Africa. This was probably due to plasmid spread, since E. coli isolates, unlike SHV-12-producing E. cloacae isolates, were not genetically related (6). The spread of resistance in this area was more likely due to plasmids carrying antibiotic resistance genes than to bacterial transmission between patients. Maintenance of these plasmids was probably favored by antibiotic pressure. These findings emphasize the need for a policy of careful antibiotic use worldwide.

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TABLE 1.

Frequency of resistance to non-β-lactam antibiotics of ESBL-producing Enterobacteriaceae isolates

ACKNOWLEDGMENTS

This work was supported by ADMIR (Association pour la Recherche en Microbiologie et Immunologie de Reims).

We are very grateful to Guillaume Arlet (Université Pierre et Marie Curie-Paris 6, France) for providing strain TN03 (E. coli O25:H4-ST 131).

  • Copyright © 2009 American Society for Microbiology

REFERENCES

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    Aibinu, I. E., V. C. Ohaegbulam, E. A. Adenipekun, F. T. Ogunsola, T. O. Odugbemi, and B. J. Mee. 2003. Extended-spectrum β-lactamase enzymes in clinical isolates of Enterobacter species from Lagos, Nigeria. J. Clin. Microbiol.41:2197-2200.
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    Clermont, O., M. Lavollay, S. Vimont, C. Deschamps, C. Forestier, C. Branger, E. Denamur, and G. Arlet. 2008. The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup. J. Antimicrob. Chemother.61:1024-1028.
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    Marcadé, G., C. Deschamps, A. Boyd, V. Gautier, B. Picard, C. Branger, E. Denamur, and G. Arlet. 2009. Replicon typing of plasmids in Escherichia coli producing extended-spectrum beta-lactamases. J. Antimicrob. Chemother.63:67-71.
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    Ruppé, E., P.-L. Woerther, A. Diop, A.-M. Sene, A. Da Costa, G. Arlet, A. Andremont, and B. Rouveix. 2009. Carriage of CTX-M-15-producing Escherichia coli isolates among children living in a remote village in Senegal. Antimicrob. Agents Chemother.53:3135-3137.
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    Weill, F. X., J. D. Perrier-Gros-Claude, M. Demartin, S. Coignard, and P. A. Grimont. 2004. Characterization of extended-spectrum-beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal. FEMS Microbiol. Lett.238:353-358.
    OpenUrlPubMedWeb of Science
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High Prevalence of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Bamako, Mali
Véronique Duval, Ibrahim Maiga, Aminata Maiga, Thomas Guillard, Lucien Brasme, Dominique Forte, Janick Madoux, Véronique Vernet-Garnier, Christophe De Champs
Antimicrobial Agents and Chemotherapy Oct 2009, 53 (11) 4957-4958; DOI: 10.1128/AAC.00675-09

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High Prevalence of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Bamako, Mali
Véronique Duval, Ibrahim Maiga, Aminata Maiga, Thomas Guillard, Lucien Brasme, Dominique Forte, Janick Madoux, Véronique Vernet-Garnier, Christophe De Champs
Antimicrobial Agents and Chemotherapy Oct 2009, 53 (11) 4957-4958; DOI: 10.1128/AAC.00675-09
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KEYWORDS

Enterobacteriaceae
beta-lactamases

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