DOI: 10.1128/AAC.00748-09
ABSTRACT
In this study, a chromosomally encoded putative drug efflux pump of the SMR family, named AbeS, from a multidrug-resistant strain of Acinetobacter baumannii was characterized to elucidate its role in antimicrobial resistance. Expression of the cloned abeS gene in hypersensitive Escherichia coli host KAM32 resulted in decreased susceptibility to various classes of antimicrobial agents, detergents, and dyes. Deletion of the abeS gene in A. baumannii confirmed its role in conferring resistance to these compounds.
- Copyright © 2009 American Society for Microbiology
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- Article
- ABSTRACT
- Cloning of a putative SMR efflux pump gene from A. baumannii.
- Antimicrobial susceptibility of E. coli expressing abeS.
- Fluorometric efflux assay of E. coli expressing AbeS.
- Construction of an abeS null mutant in A. baumannii.
- Functional characterization of abeS in A. baumannii.
- Nucleotide sequence accession number.
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- FOOTNOTES
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