Article Figures & Data
Figures
- FIG. 1.
PFGE of the 37 isolates analyzed. The names and PFGE types of the isolates are shown at the right of the figure. The dendrogram depicting genetic relatedness and generated using BioNumerics is shown at the left of the figure. The scale for the dendrogram represents percentage of relatedness. Staphylococcus aureus strain NCTC 8325 was used for the gel-to-gel normalization required to generate the dendrogram.
Tables
- TABLE 1.
Characteristics of the P. aeruginosa isolates from Puerto Rico
Strain Sample site Hospitala Hospital wardb PFGE group MIC (μg/ml)c PCR resultd pI Sequence analysise Expression off: OprD productiong P/T CAZ IPM MEM IMP VIM KPC OXA mexA oprD PAO1 4 1 1 0.5 1.0 1.0 + PS1 Sputum 1 General 9C 8 2 16 8 Neg Neg Neg 4.2 0.008 PS2 Urine 1 ICU 10B 4 128 64 16 Pos Neg Neg Pos 8.0 IMP-18, OXA-2 PS3 Sputum 2 ICU 12 8 4 16 8 Neg Neg Neg 1.0 0.002 − PS4 Sputum 3 General 9B 64 >128 >64 64 Pos Neg Neg Neg 5.4, 7.5, ≥8.5 IMP-18 PS5 Sputum 1 ICU 7A >256 64 >64 >64 Neg Neg Pos Neg 6.7, ≥8.5 KPC-2 1.8 0.005 − PS6A Sputum 4 ICU 11A >256 32 >64 >64 Neg Neg Pos 1.8 0.0005 − PS7A Urine 4 ICU 11D >256 64 >64 >64 Neg Neg Pos PS9 Sputum 1 General 3B 64 8 8 16 Neg Neg Neg PS10 Blood 1 General 1A >256 64 >64 >64 Neg Neg Pos Neg 6.7, ≥8.5 KPC-2 1.5 0.003 − PS11A Sputum 1 ICU 7A >256 32 >64 >64 Neg Neg Pos PS12 Sputum 1 General 7B >256 64 >64 32 Neg Neg Pos PS13 Sputum 1 ICU 11E >256 64 >64 >64 Neg Neg Pos PS14 Sputum 1 ICU 10C 4 64 64 16 Pos Neg Neg PS15A Blood 1 General 10A 8 128 32 16 Pos Neg Neg PS17B Blood 1 ICU 11D >256 64 >64 >64 Neg Neg Pos Neg 6.7, ≥8.5 KPC-2 2.0 0.0004 − PS18A CSF 1 ICU 11D >256 32 >64 >64 Neg Neg Pos PS19A Urine 1 General 11C >256 64 >64 >64 Neg Neg Pos PS20A Skin 5 General 11D >256 64 >64 >64 Neg Neg Pos 1.9 0.0003 − PS21 Skin 4 General 11F >256 64 >64 >64 Neg Neg Pos PS22 Urine 4 General 3A 256 16 8 16 Neg Neg Neg PS23 Blood 1 General 4A >256 128 >64 >64 Neg Neg Pos 6.7, ≥8.5 KPC-2 PS25 Sputum 1 General 1A >256 64 >64 >64 Neg Neg Pos PS26 Sputum 3 ICU 3A 128 8 8 16 Neg Neg Neg Pos 7.5, 8.0 OXA-2 PS28 Skin 5 General 6 128 128 >64 >64 Neg Neg Pos 7.65, ≥8.5 KPC-5 PS29 Sputum 4 General 11D >256 64 >64 >64 Neg Neg Pos PS30 Sputum 4 General 11D >256 64 >64 >64 Neg Neg Pos PS31 Skin 4 General 11B >256 64 >64 >64 Neg Neg Pos PS33 Blood 1 General 9A 32 >128 64 32 Pos Neg Neg PS34 Blood 1 ICU 4A >256 64 >64 >64 Neg Neg Pos PS35 Urine 4 General 11A >256 64 >64 >64 Neg Neg Pos PS36 Urine 4 General 11B >256 64 >64 >64 Neg Neg Pos PS37 Sputum 4 General 11D >256 64 >64 >64 Neg Neg Pos PS39 Urine 1 General 8 128 >128 >64 64 Pos Neg Neg Neg 5.4, 6.0, 7.5, ≥8.5 IMP-18 4.1 0.015 − PS40 Urine 6 General 5 128 32 >64 >64 Neg Neg Pos Pos 6.7, 8.0, ≥8.5 KPC-2, OXA-2 PS41 Urine 4 General 11D >256 64 >64 >64 Neg Neg Pos PS43 Skin 1 ICU 2 256 16 16 32 Neg Neg Pos Pos 6.7, 8.0, ≥8.5 KPC-2, OXA-2 1.8 0.56 − PS44 Blood ER NR 13 32 >128 64 32 Pos Neg Neg Neg 5.4, 6.0, 7.5, ≥8.5 IMP-18 3.3 0.36 − ↵a ER, emergency room.
↵b ICU, intensive care unit (includes medical, surgical, pediatric, and neurosurgical units); NR, not reported.
↵c P/T, piperacillin-tazobactam; CAZ, ceftazidime; IPM, imipenem; MEM, meropenem.
↵d Neg, negative for amplification; Pos, positive for amplification.
↵e The blaKPC and blaIMP-18 sequence represents the entire structural gene. The blaOXA-2 sequence was obtained from 555 bp of the amplified fragment.
↵f Expression data are presented as fold differences compared to the wild-type strain PAO1.
↵g OprD production was assessed by outer membrane analysis.
