Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Antimicrobial Agents and Chemotherapy
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mechanisms of Action: Physiological Effects

Kinetic Parameters of Efflux of Penicillins by the Multidrug Efflux Transporter AcrAB-TolC of Escherichia coli

Siew Ping Lim, Hiroshi Nikaido
Siew Ping Lim
Department of Molecular and Cell Biology, University of California, Berkeley, California
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroshi Nikaido
Department of Molecular and Cell Biology, University of California, Berkeley, California
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: nhiroshi@berkeley.edu
DOI: 10.1128/AAC.01714-09
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Ves (circles) and Vhs (triangles) of isoxazolylpenicillins in strain HN1157/pHSGoxa7. The rates were plotted against Cp, determined from the rates of hydrolysis in intact cells, as described in the text. Top, dicloxacillin; middle, cloxacillin; bottom, oxacillin.

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    Ves (circles) and Vhs (triangles) of ureidopenicillins in strain HN1157/pHSGoxa7. The rates were plotted against Cp, determined from the hydrolysis rates in intact cells, as described in the text. Top, azlocillin; middle, mezlocillin; bottom, piperacillin.

  • FIG. 3.
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    Ves (circles) and Vhs (triangles) of penicillin V and ampicillin in strain HN1157/pHSGoxa7. The rates were plotted against Cp, determined from the hydrolysis rates in intact cells, as described in the text. Top, penicillin V; bottom, ampicillin.

Tables

  • Figures
  • TABLE 1.

    Kinetic parameters of OXA-7 enzyme

    Substrate% glucose used for growth$$mathtex$$\(\mathit{V}^{\mathit{max}}\)$$mathtex$$ (nmol/mg/s)aKm (μM)a
    Dicloxacillin0.15.934
    Cloxacillin0.16.331
    Oxacillin0.19.352
    Azlocillin04.318
    Mezlocillin05.029
    Piperacillin03.534
    Penicillin V0.16.729
    Ampicillin0.14.121.5
    • ↵ a The values shown are the averages of three independent experiments. $$mathtex$$\(\mathit{V}^{\mathit{max}}\)$$mathtex$$ is the maximal rate of hydrolysis.

  • TABLE 2.

    Kinetic parameters of the efflux process

    Substrate$$mathtex$$\(\mathit{V}_{\mathit{e}}^{\mathit{max}}\)$$mathtex$$ (nmol/mg/s)Hill coefficientK0.5 (μM)Permeability coefficient (μm/s)Substituent log Pa
    Dicloxacillin0.98, 0.95, 0.943.6, 4.7, 4.30.53, 0.83, 0.820.6, 0.6, 0.63.4
    Cloxacillin0.89, 0.99, 0.802.3, 2.7, 2.40.95, 1.03, 0.910.6, 0.6, 0.62.8
    Oxacillin0.94, 1.1, 0.942.6, 2.7, 2.61.04, 0.97, 1.030.6, 0.6, 0.62.1
    Azlocillin0.42, 0.41, 0.354.0, 4.0, 4.61.08, 1.06, 1.090.4, 0.4, 0.40.71
    Mezlocillin0.51, 0.47, 0.504.4, 4.5, 4.51.09, 1.08, 1.110.5, 0.5, 0.50.67
    Piperacillin0.39, 0.35, 0.394.1, 3.7, 4.11.06, 1.08, 1.080.4, 0.4, 0.30.45
    Penicillin V0.72, 0.85, 0.724.7, 4.7, 4.60.82, 0.84, 0.830.8, 0.7, 0.71.54
    Ampicillin0.38, 0.37, 0.371.9, 2.3, 2.50.88, 0.92, 1.030.8, 0.8, 0.8−1.03
    • ↵ a The log P values of the 6-substituent were calculated after the carboxyl group was changed to a methyl group at the website http://www.molinspiration.com/services/lopP.html .

  • TABLE 3.

    Predicted and measured MICs for HN1157/pHSGoxa7

    PenicillinCinh (μg/ml)MIC (μg/ml)
    PredictedMeasured
    Dicloxacillin18.4 (39)a285560
    Cloxacillin4.9 (11)147295
    Oxacillin4.8 (12)135282
    Piperacillin0.6 (1.2)3046
    Azlocillin0.6 (1.3)4044
    Mezlocillin0.6 (1.1)3330
    Penicillin V11.5 (33)181178
    Ampicillin1.0 (2.9)2639
    • ↵ a The numbers in parentheses are in μM.

PreviousNext
Back to top
Download PDF
Citation Tools
Kinetic Parameters of Efflux of Penicillins by the Multidrug Efflux Transporter AcrAB-TolC of Escherichia coli
Siew Ping Lim, Hiroshi Nikaido
Antimicrobial Agents and Chemotherapy Apr 2010, 54 (5) 1800-1806; DOI: 10.1128/AAC.01714-09

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Antimicrobial Agents and Chemotherapy article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Kinetic Parameters of Efflux of Penicillins by the Multidrug Efflux Transporter AcrAB-TolC of Escherichia coli
(Your Name) has forwarded a page to you from Antimicrobial Agents and Chemotherapy
(Your Name) thought you would be interested in this article in Antimicrobial Agents and Chemotherapy.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Kinetic Parameters of Efflux of Penicillins by the Multidrug Efflux Transporter AcrAB-TolC of Escherichia coli
Siew Ping Lim, Hiroshi Nikaido
Antimicrobial Agents and Chemotherapy Apr 2010, 54 (5) 1800-1806; DOI: 10.1128/AAC.01714-09
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS AND DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Bacterial Outer Membrane Proteins
Drug Resistance, Bacterial
Escherichia coli
Escherichia coli Proteins
Membrane Transport Proteins
Multidrug Resistance-Associated Proteins
penicillins

Related Articles

Cited By...

About

  • About AAC
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • AAC Podcast
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AACJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0066-4804; Online ISSN: 1098-6596