Article Figures & Data
Figures
- FIG. 1.
Changes in the index of resistance (ratio of the IC50 for the resistant progeny to the IC50 for the parental line) to various drugs during the adaptation of the Plasmodium falciparum lines W2 (A), TM91C235 (B), and D6 (C) to artelinic acid (AL).
- FIG. 2.
pfmdr1 copy numbers in parental parasites and resistant progenies.
- FIG. 3.
Southern blot hybridization of P. falciparum chromosomes digested with SmaI (left) and BglI (right) and hybridized with probes derived from the pfmdr1 (A) and aldolase (B) genes.
- FIG. 4.
Transcription of the pfmdr1 gene in the W2, W2AL80, W2QHS40, TM91C235, TM91C235AL80, D6, and D6AL80 lines of P. falciparum.
- FIG. 5.
MDR1 protein expression in resistant and parental Plasmodium falciparum lines. Western blot hybridization was performed with anti-Pgh1 (α1435; left side in panels A and B) and anti-RAP2 (6C1; right side in panels A and B) antibodies.
Tables
- TABLE 1.
In vitro susceptibilities of Plasmodium falciparum lines to artelinic acid
Parasite line Mean IC (nM) ± SDa 50% 90% W2 4.73 ± 0.19 7.45 ± 0.40 W2AL50 23.30 ± 3.95 51.31 ± 1.31 W2AL80 22.75 ± 5.37 56.80 ± 5.44 D6 9.81 ± 1.41 21.83 ± 3.04 D6AL50 34.00 ± 3.14 81.26 ± 8.67 D6AL80 23.64 ± 3.71 75.89 ± 2.60 TM91C235 11.08 ± 1.68 22.99 ± 2.60 TM91C235AL50 33.72 ± 1.64 53.51 ± 1.12 TM91C235AL80 24.55 ± 4.13 75.89 ± 6.8 ↵ a n, ≥3.
- TABLE 2.
pfmdr1 allelic types in parental and resistant Plasmodium falciparum lines
Parasite line Amino acid at position: 86 184 1034 1042 1246 D6 N Y S N D D6AL80 N Y S N D TM91C235 N F S N D TM91C235AL80 N F S N D W2 Y Y S N D W2AL200 Y Y S N D W2QHS200 Y Y S N D W2AS80 Y Y S N D
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
Table S1, Primers used for PCR amplification, sequencing, and real-time PCR. Table S2, information on the repetitive region
in PfUBCTH.
Zipped MS Word document, 25K.
- Supplemental file 1
-
Table S1, Primers used for PCR amplification, sequencing, and real-time PCR. Table S2, information on the repetitive region
in PfUBCTH.
