Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Antimicrobial Agents and Chemotherapy
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mechanisms of Resistance

SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1 and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate

Jun-ichi Wachino, Hiroyuki Yoshida, Kunikazu Yamane, Satowa Suzuki, Mari Matsui, Takuya Yamagishi, Atsuko Tsutsui, Toshifumi Konda, Keigo Shibayama, Yoshichika Arakawa
Jun-ichi Wachino
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: wachino@nih.go.jp
Hiroyuki Yoshida
2Department of Infection Control and Prevention, Kobe University Hospital, 7-5-2 Chuo-ku, Kobe, Hyogo 650-0017, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kunikazu Yamane
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
3Department of Public Health, Kawasaki Medical University, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Satowa Suzuki
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mari Matsui
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takuya Yamagishi
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Atsuko Tsutsui
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toshifumi Konda
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Keigo Shibayama
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshichika Arakawa
1Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
4Department of Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/AAC.05045-11
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Amino acid alignments of SMB-1 sequence with those of other subclass B3 MBLs. The residues involved in zinc binding are highlighted with a dark background. The signal peptide of SMB-1 is shown with an underline. An asterisk indicates amino acid residues conserved among all subclass B3 MBLs. Proteins (GenBank accession no.) are the following: SMB-1 (AB636283), AMO1 (ACS83721), BJP-1 (NP772870), CAU-1 (CAC87665), CRB11 (ACS83724), FEZ-1 (CAB96921), GOB-1 (ABO21417), L1 (ABO60992), POM-1 (ADC79555), THIN-B (CAC33832), and CAR-1 (Q6D395).

  • Fig. 2.
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Tree view exhibiting the similarity of SMB-1 with the other subclass B3 MBLs. The tree was constructed using ClustalW, version 1.83 (http://clustalw.ddbj.nig.ac.jp/top-j.html), and was provided by the DNA Data Bank of Japan (DDBJ). Sequences incorporated to draw the tree were the same as those used for Fig. 1. The 0.1 scale represents a genetic unit reflecting 10% of the amino acid substitutions, and it was calculated with the ClustalW program.

  • Fig. 3.
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Localization of the blaSMB-1 gene on I-CeuI-digested total DNA of S. marcescens strain 10mdr148 separated by PFGE. Lane: M, CHEF DNA size standard marker (Bio-Rad); A, I-CeuI-digested total DNA of S. marcescens strain 10mdr148 stained with ethidium bromide; B, hybridization of I-CeuI-digested total DNA of S. marcescens strain 10mdr148 with probe specific for 16S rRNA gene; and C, hybridization of I-CeuI-digested total DNA of S. marcescens strain 10mdr148 with probe specific for the blaSMB-1 gene.

  • Fig. 4.
    • Open in new tab
    • Download powerpoint
    Fig. 4.

    Schematic representation of the generic environment of the blaSMB-1 gene. The 5′ end of the qacEΔ1 gene in the second 3′-CS was deleted of 143 bp that are present in the first 3′-CS.

Tables

  • Figures
  • Table 1.

    Primers used in this study

    PrimerSequenceaNucleotide positionb
    ampC-F5′-ATG ACG AAA GTG AAC CGC-3′1-18
    ampC-R5′-CCT GGA CGA TGT GGT AAG-3′1103-1120
    CTX-M-3F5′-ACC GTC ACG CTG TTG TTA G-3′47-64
    CTX-M-3R5′-CTT TCT GCC TTA GGT TGA G-3′806-824
    IMP-1F5′-ACC GCA GCA GAG TCT TTG CC-3′49-68
    IMP-1R5′-ACA ACC AGT TTT GCC TTA CC-3′616-635
    IMP-2F5′-GTT TTA TGT GTA TGC TTC C-3′16-34
    IMP-2R5′-AGC CTG TTC CCA TGT AC-3′677-693
    VIM-2F5′-ATG TTC AAA CTT TTG AGT AAG-3′1-21
    VIM-2R5′-CTA CTC AAC GAC TGA GCG-3′784-801
    NDM-1F5′-TTG CCC AAT ATT ATG CAC CC-3′7-26
    NDM-1R5′-ATT GGC ATA AGT CGC AAT CC-3′407-426
    16S rRNA-10F5′-GTT TGA TCC TGG CTC A-3′11-26
    16S rRNA-800R5′-TAC CAG GGT ATC TAA TCC-3′785-802
    SMB-F5′-CAG CAG CCA TTC ACC ATC TA-3′79-98
    SMB-R5′-GAA GAC CAC GTC CTT GCA CT-3′551-570
    SMB-CloF5′-CCC AAG CTT TCC GCC GAC TTG GCG CAG-3′
    SMB-CloR5′-GGG GTA CCA AGA CCG ATT TAG CCG GC-3′
    PET-15′-GGA ATT CCA TAT GAA AAT CAT CGC TTC CC-3′
    PET-25′-CCC AAG CTT TCA GCG TTT CTC GCT GGC C-3′
    • ↵a Underlines indicate the sites for restriction endonuclease.

    • ↵b Position numbers correspond to the nucleotides of the coding sequences. Position numbers are assigned to the primers that amplify the internal region of the coding sequences.

  • Table 2.

    Result of susceptibility testing

    Antimicrobial agentMIC (μg/ml) against strain:
    S. marcescens 10mdr148E. coli KAM32 (pCL-SMB)E. coli KAM32 (pCL1920)
    Ampicillin>256>2562
    Piperacillin>256>2560.25
    Cephalothin>2562568
    Cephaloridine>256642
    Cefazolin>2562561
    Cefuroxime>256>2560.25
    Cefotaxime>25616≤0.06
    Ceftazidime2562560.25
    Cefepime>2560.5≤0.06
    Cefoxitin>256>2562
    Cefmetazole>2562560.5
    Flomoxef>25664≤0.06
    Aztreonam>2560.130.13
    Imipenem>3280.25
    Meropenem>32160.03
    Panipenem>32320.5
    Biapenem>32160.25
    Amikacin12NDaND
    Gentamicin1.5NDND
    Ciprofloxacin1NDND
    Moxifloxacin0.5NDND
    Tigecycline1NDND
    • ↵a ND, not determined.

  • Table 3.

    Kinetic parameters and inhibition profile of SMB-1a

    Substrate or chelating agentKm (μM)kcat (s−1)kcat/Km (M−1·s−1)Relative kcat/KmdIC50 (μM)
    Ampicillin1022472.4 × 106100
    Piperacillin380681.8 × 1057.5
    Cephalothin15281.9 × 10679
    Cefuroxime22301.4 × 10658
    Cefotaxime35318.9 × 10537
    Ceftazidime574.47.7 × 1043.2
    Cefepime7472.73.6 × 1030.15
    Cefoxitin26391.5 × 10663
    AztreonamNHbNDcNDND
    Imipenem1335183.9 × 106163
    Meropenem1446044.2 × 106175
    Dipicolinic acid2.2
    1,10-o-Phenanthroline156
    EDTA14
    • ↵a Standard deviations for each parameter were below 10%.

    • ↵b NH, no measurable hydrolysis detected with 1 μM enzyme.

    • ↵c ND, not determined.

    • ↵d Relative kcat/Km value was expressed compared to that of ampicillin, which was assigned 100.

PreviousNext
Back to top
Download PDF
Citation Tools
SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1 and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate
Jun-ichi Wachino, Hiroyuki Yoshida, Kunikazu Yamane, Satowa Suzuki, Mari Matsui, Takuya Yamagishi, Atsuko Tsutsui, Toshifumi Konda, Keigo Shibayama, Yoshichika Arakawa
Antimicrobial Agents and Chemotherapy Oct 2011, 55 (11) 5143-5149; DOI: 10.1128/AAC.05045-11

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Antimicrobial Agents and Chemotherapy article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1 and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate
(Your Name) has forwarded a page to you from Antimicrobial Agents and Chemotherapy
(Your Name) thought you would be interested in this article in Antimicrobial Agents and Chemotherapy.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1 and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate
Jun-ichi Wachino, Hiroyuki Yoshida, Kunikazu Yamane, Satowa Suzuki, Mari Matsui, Takuya Yamagishi, Atsuko Tsutsui, Toshifumi Konda, Keigo Shibayama, Yoshichika Arakawa
Antimicrobial Agents and Chemotherapy Oct 2011, 55 (11) 5143-5149; DOI: 10.1128/AAC.05045-11
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS AND DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About AAC
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • AAC Podcast
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AACJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0066-4804; Online ISSN: 1098-6596