Ribosomal Mutations as the Main Cause of Macrolide Resistance in Campylobacter jejuni and Campylobacter coli

DOI: 10.1128/AAC.00314-11

Article Figures & Data

Table 1.

Ribosomal mutations and erythromycin susceptibility in Campylobacter jejuni strains compared to C. jejuni DSM 4688 type strain^e

Strain(s)	Erythromycin MIC (μg/ml)^d	23S rRNA gene mutation^a	L4 mutation(s)	L22 mutation(s)	L22 insertion
DSM 4688^b	0.5
1	>128	wt	A121V, I192M	A74G, A109T, E111A, T114A, A130T, A132V, A141V, K144E	73-GAVLT-74
2–6	>128	A2059G	A121V, I192M	A74G, A109T, E111A, T114A, A130T, A132V, A141V, K144E
7	>128	A2059G	wt	A74G, A103V, A141V, K144E
8^c	>128	A2059G	wt	A74G, A103V, A141V
9, 10	>128	A2059G	A121V, I192M, A196V	V65I, A109S, A143V
11	>128	A2059G	A121V, I192M	Q24R, V65I, A143V
12	>128	A2059G	A121V, I192M	V65I, A103V
13	>128	A2059G	A121V, I192M	G86E
14	128	A2059G	A121V, I192M	A74G, A109T, E111A, T114A, A130T, A132V, A141V, K144E
15	128	A2059G	wt	V65I, A103V
16	64	wt	T177S	I65V, D72N
17, 18	8	wt	A121V, I192M	Q24R, V65I, A143V
19	8	wt	A121V, I192M	V65I, A109S, A143V
20, 22	4–8	wt	A121V, I192M	V65I, A103V
21	4	wt	N95S, A121V, I192M	Q24R, V65I, A143V
23	2	wr	A129T, T201I	wt
24, 25	1	wt	A121V, I192M	V65I, A109S, A143V
26, 30, 31	0.5–1	wt	A121V, I192M, A196V	V65I, A109S, A143V
27	1	wt	wt	V65I, A103V
28, 29	1	wt	T177S	V65I
32	0.5	wt	V80I, A121V, I192M	V65I, A103V
33	0.5	wt	S132N, T177S	V65I
34	0.5	wt	A140T	wt

↵a Position according to Escherichia coli numbering.
↵b Reference strain for the ribosomal proteins L4 and L22.
↵c Only two mutated copies of A2059G.
↵d From each MIC, a number of strains were selected for the efflux pump inhibitor (NMP and PAβN) testing, and no decrease in MIC was observed.
↵e Abbreviations: A, Ala; D, Asp; E, Glu; G, Gly; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; wt, wild type, based on corresponding sequences of C. jejuni DSM 4688 type strain.

Table 2.

Ribosomal mutations and erythromycin susceptibility in Campylobacter coli strains compared to C. coli DSM 4689 type strain^e

Strain(s)	Erythromycin MIC (μg/ml)	23S rRNA gene mutation^a	L4 mutation(s)	L22 mutation(s)	L22 insertions
DSM 4689^b	0.5	wt
36	>128	A2059G	S28P, T176S	wt
41,^c 37–40, 42–44	>128	A2059G	S28P	wt
45	>128	A2059G	S28P, V121A, M192I	wt
46, 47	>128	A2059G	S28P, V121A, V176I, T177S, V184I, M192I	A103V
48	>128	A2059G	S28P	G58S
49	>128	A2059G	S28P, M192I	T95I, A103V, T109A, A111E, A114T, V121A	119-TTTKA-120, 124-T-125
50	>128	A2059G	S28P, M192I	A103V, T109A, A111E, A114T, V121A	119-TTTKA-120, 124-T-125
51^d	128	A2059G	S28P, V121A, M192I	A103V, T109A, A111E, A114T, V121A	119-TTTKA-120, 124-T-125
52	16	wt	S28P	wt
53	8	wt	S28P, V121A, V176I, T177S, V184I, M192I	A103V
54	8	wt	S28P, M192I	A103V, T109A, A111E, A114T, V121A	119-TTTKA-120, 124-T-125

↵a Position according to Escherichia coli numbering.
↵b Reference strain for the ribosomal proteins L4 and L22.
↵c Only two mutated copies of A2059G.
↵d NMP caused 2-fold decrease in MIC.
↵e Abbreviations: A, Ala; E, Glu; G, Gly; I, Ile; K, Lys; M, Met; P, Pro; S, Ser; T, Thr; V, Val; wt, wild type, based on corresponding sequences of C. coli DSM 4689 type strain. One strain from each row was selected for the efflux pump inhibitor (NMP and PAβN) testing, and only one strain exhibited a 2-fold decrease in MIC.