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Epidemiology and Surveillance

Rapid Identification of Carbapenemase Types in Enterobacteriaceae and Pseudomonas spp. by Using a Biochemical Test

Laurent Dortet, Laurent Poirel, Patrice Nordmann
Laurent Dortet
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France
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Laurent Poirel
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France
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Patrice Nordmann
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France
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DOI: 10.1128/AAC.01395-12
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ABSTRACT

A biochemical test (Carba NP test II) was developed to identify carbapenemase production in Enterobacteriaceae and Pseudomonas spp. and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of the acidification resulting from imipenem hydrolysis, coupled with tazobactam and EDTA as inhibitors. This is an easy and reliable technique (100% sensitivity and specificity) for detection of not only carbapenemase activity but also carbapenemase types in Enterobacteriaceae and Pseudomonas aeruginosa.

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Rapid Identification of Carbapenemase Types in Enterobacteriaceae and Pseudomonas spp. by Using a Biochemical Test
Laurent Dortet, Laurent Poirel, Patrice Nordmann
Antimicrobial Agents and Chemotherapy Nov 2012, 56 (12) 6437-6440; DOI: 10.1128/AAC.01395-12

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Rapid Identification of Carbapenemase Types in Enterobacteriaceae and Pseudomonas spp. by Using a Biochemical Test
Laurent Dortet, Laurent Poirel, Patrice Nordmann
Antimicrobial Agents and Chemotherapy Nov 2012, 56 (12) 6437-6440; DOI: 10.1128/AAC.01395-12
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