DOI: 10.1128/AAC.05785-11
Article Figures & Data
Figures
- Fig 1
(A) Mean plasma concentration profiles of BNZ administered alone and in association with ITC in mice (n = 5) after a single oral dose of 5 mg of each drug. (B) Highlight of the final portion of the curve displayed in panel A.
- Fig 2
Mean plasma concentration profiles of ITC administered alone and in association with BNZ in mice (n = 5) after a single oral dose of 5 mg of each drug.
Tables
- Table 1
Validation parameters of the HPLC-UV method developed for simultaneous quantification of BNZ and ITC in plasma of mice (mean, 5 repetitions)
Validation parameter Value BNZ ITC Sensitivity Limit of quantification (μg/ml) 0.5 1.0 Linearity Correlation coefficient 0.9985 0.9924 Mean regression equation y = 0.0522x + 0.0229 y = 0.0128x + 0.0111 Reproducibility Mean accuracy (%)b 96.67 93.37 Mean precision (RSD) (%)a 3.87 5.43 Recovery Mean recovery (%) 92.46 93.92 - Table 2
Pharmacokinetic parameters of BNZ and ITC administered alone and in association in mice after a single oral dose of 5 mg of each drug
Parameter Valuea when administered: Alone In association BNZ ITC BNZ ITC Cmax (μg/kg) 151.28 ± 6.03b 11.76 ± 1.33 101.19 ± 5.10b 10.55 ± 0.96 Tmax (h) 2.2 ± 0.2 4.6 ± 0.24 2.4 ± 0.24 5.0 ± 0.32 AUC0-t (μg · h/ml) 539.14 ± 14.54 65.93 ± 2.99 612.33 ± 50.87 77.45 ± 9.17 AUC0-∞ (μg · h/ml) 547.53 ± 11.96 79.51 ± 4.69 673.90 ± 64.24 94.21 ± 12.61 AUMC0-t (μg · h2/ml) 2,635.39 ± 65.66b 526.67 ± 41.65 7,465.97 ± 1,345.0b 949.05 ± 231.0 AUMC0-∞ (μg · h2/ml) 2,757.49 ± 86.19b 753.16 ± 74.53 10,817.19 ± 2,112.0b 1,377.09 ± 386.6 MRT (h) 5.05 ± 0.22b 9.38 ± 0.42 15.47 ± 1.7b 13.52 ± 2.17 CL (ml/h/kg) 416.74 ± 10.92 3,417.30 ± 149.50 375.2 ± 28.9 3,077.97 ± 404.4 V (ml/kg) 2,112.33 ± 136.90b 31,870.60 ± 970.80 5,621.29 ± 209.5b 38,710.66 ± 2,604.0 λz (1/h) 0.51 ± 0.09b 0.23 ± 0.04 0.05 ± 0.00b 0.21 ± 0.04 t½β (h) 1.64 ± 0.41b 3.39 ± 0.47 12.18 ± 0.78b 3.89 ± 0.79
