DOI: 10.1128/AAC.00726-13
ABSTRACT
In this study, we attempted to adopt the auxotrophic mevalonate synthase mutant (ΔmvaS mutant) of Staphylococcus aureus to study whether a nongrowing but viable cell population is tolerant to bactericidal antibiotics. The mevalonate-depleted nongrowing ΔmvaS mutant was found tolerant to antibiotics. Surprisingly, after prolonged cultivation, we obtained stable ΔmvaS variants that were able to grow without mevalonate, which suggested unknown mechanisms for compensating undecaprenyl pyrophosphate production without mevalonate in S. aureus.
FOOTNOTES
- Received 10 April 2013.
- Returned for modification 18 May 2013.
- Accepted 30 July 2013.
- Accepted manuscript posted online 19 August 2013.
Supplemental material for this article may be found at http://dx.doi.org/10.1128/AAC.00726-13.
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