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Mechanisms of Resistance

Complete Sequence of a blaKPC-2-Harboring IncFIIK1 Plasmid from a Klebsiella pneumoniae Sequence Type 258 Strain

Liang Chen, Kalyan D. Chavda, Roberto G. Melano, Michael R. Jacobs, Michael H. Levi, Robert A. Bonomo, Barry N. Kreiswirth
Liang Chen
aPublic Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
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Kalyan D. Chavda
aPublic Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
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Roberto G. Melano
bOntario Agency for Health Protection and Promotion, Toronto, Ontario, Canada
cUniversity of Toronto, Toronto, Ontario, Canada
dMount Sinai Hospital, Toronto, Ontario, Canada
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Michael R. Jacobs
eDepartment of Pathology, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA
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Michael H. Levi
fDivision of Pathology, Montefiore Medical Center and the Albert Einstein College of Medicine, Bronx, New York, USA
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Robert A. Bonomo
gResearch Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA
hDepartments of Medicine, Pharmacology, Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA
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Barry N. Kreiswirth
aPublic Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
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DOI: 10.1128/AAC.02332-12
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    Fig 1

    Comparative analysis of IncFIIK plasmids pKPN3 (CP000648), pKPN4 (CP000649), pKpQIL (GU595196), and pBK32179 (JX430448). Light blue shading denotes shared regions of homology (>99.5% nucleotide similarity). Open reading frames (ORFs) are indicated by arrows and colored based on predicted gene function. Orange arrows indicate plasmid scaffold regions. The genes associated with the tra locus are indicated by green arrows, and replication-associated genes are denoted as dark blue arrows. Antimicrobial resistance genes are indicated by red arrows, while copper, mercury, and silver resistance genes are shown by light blue arrows. Other genes in the accessory region are indicated by yellow arrows. Small black arrowheads beneath the plasmids indicate the locations of primers used for PCR screening of pBK32179-like plasmids. Primer sequences used for PCRs were as follows: 1, repA-F (CTTCACGTCCCGTTTTGATT), 2, repA-R (CGCTTCAGCGCTTCTTTATC) (657 bp); 3, hel-F (ATGGTGGGGGAAAAATTCAT), 4, 4401-R (GTCACGTCAAGCGAGGAGTC) (1,996 bp); 5, repA2-F (ACGTTAAGATCACCGGTTCG), and 6, repA2-R (TAGGCTGACTGCACCAGATG) (271 bp). pBK32179-like plasmids are positive by targets of primer sets 1/2, 3/4, and 5/6, while pKpQIL-, pKPN4-, and pKPN3-like plasmids are positive only by PCRs of primer sets 1/2 and/or 5/6 but not set 3/4.

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Complete Sequence of a blaKPC-2-Harboring IncFIIK1 Plasmid from a Klebsiella pneumoniae Sequence Type 258 Strain
Liang Chen, Kalyan D. Chavda, Roberto G. Melano, Michael R. Jacobs, Michael H. Levi, Robert A. Bonomo, Barry N. Kreiswirth
Antimicrobial Agents and Chemotherapy Feb 2013, 57 (3) 1542-1545; DOI: 10.1128/AAC.02332-12

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Complete Sequence of a blaKPC-2-Harboring IncFIIK1 Plasmid from a Klebsiella pneumoniae Sequence Type 258 Strain
Liang Chen, Kalyan D. Chavda, Roberto G. Melano, Michael R. Jacobs, Michael H. Levi, Robert A. Bonomo, Barry N. Kreiswirth
Antimicrobial Agents and Chemotherapy Feb 2013, 57 (3) 1542-1545; DOI: 10.1128/AAC.02332-12
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