Article Figures & Data
Figures
- FIG 1
Characteristics of the 46 fusidic acid-resistant MRSA isolates. The dendrogram was generated by using BioNumerics software with the Dice similarity coefficient and unweighted-pair group method (UPGMA) based on the PFGE profiles. The degree of similarity is shown in the scale. To the right are shown Fus gene determinants for resistance to fusidic acid (A, fusA point mutation; C, fusC determinant), SCCmec types (VT, VT), spa types, fusidic acid MICs (μg/ml), and MLST results for selected isolates representing each spa type.
- FIG 2
Genetic organization of SCCfusC (GenBank accession no. KF527883) compared with that of SCC476 (GenBank accession no. NC_002953.3). The patterned arrows represent the putative open reading frames. The genes are drawn according to their sequences and function. hsdR, hsdM, and hsdS encode the R, M, and S subunits of the type I restriction-modification system. speG encodes spermidine acetyltransferase. White arrows represent other genes encoding hypothetical proteins. The integration site sequences (ISS) are indicated by the vertical arrows. The inverted repeat sequences are indicated by the thin horizontal arrows. The homologous regions between the two SCCs are shown by shading, and the numbers in the shading indicate the percentages of homology between the corresponding sequences. The horizontal bars at the bottom represent the PCR products used to confirm the presence of the SCCfusC element. The identities of hsdR, hsdM, and hsdS between the two elements were below 45%.
Tables
- TABLE 1
Distribution of fusidic acid MIC and resistance determinants among the fusidic acid-resistant MRSA isolates
Resistance determinant (no. of isolates with determinant) No. of isolates with fusidic acid MICs (μg/ml) of: 2–16 32–64 ≥128 fusA mutation (19) 4 5 10 fusC (27) 27 0 0 Total (46) 31 5 10
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Tables S1 and S2.
PDF, 51K
- Supplemental file 1 -
