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Epidemiology and Surveillance

Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom

Dorina Timofte, Iuliana E. Maciuca, Nicholas J. Evans, Helen Williams, Andrew Wattret, Jenny C. Fick, Nicola J. Williams
Dorina Timofte
aSchool of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom
bFaculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Iasi, Romania
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Iuliana E. Maciuca
bFaculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Iasi, Romania
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Nicholas J. Evans
cInstitute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom
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Helen Williams
aSchool of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom
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Andrew Wattret
aSchool of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom
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Jenny C. Fick
aSchool of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom
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Nicola J. Williams
cInstitute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom
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DOI: 10.1128/AAC.00752-13
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    FIG 1

    Schematic representations of environments surrounding blaCTX-M-15 in common human isolates (a to c) and bovine mastitis isolate 26 reported here (d). Where known, elements are qualified as partial or complete (due to primer locations, in some cases elements cannot be classified as either partial or complete).

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  • TABLE 1

    Antimicrobial susceptibility profiles of bovine mastitis E. coli and Klebsiella pneumoniae subsp. pneumoniae isolates and selected transconjugantsa

    AntimicrobialMIC (μg/ml)b
    Parental isolatesConjugants
    25(2053)26(2104A)27(2104B)28(2104C)32(2188)26C(2104A)28C(2104C)32C(2188)
    AMP323232323232432
    CFZ323232323232432
    FEP24848828
    CTX432321664320.1232
    CTX-CLA0.250.060.50.50.2540.064
    FOX1282128128212816128
    CPD646464646464164
    CAZ646425612864828
    CAZ-CLA0.50.060.50.50.2520.252
    CRO16646432128640.564
    CEF323232323232NM32
    CIP0.50.50.50.50.540.5NM
    GEN323216163216216
    IPM0.250.250.250.250.250.250.250.25
    MEM0.50.50.50.50.50.50.50.5
    PIP-TZB2212882222
    • ↵a Abbreviations: AMP, ampicillin; CFZ, cefazolin; FEP, cefepime; CTX, cefotaxime; CTX-CLA, cefotaxime-clavulanic acid; FOX, cefoxitin; CPD, cefpodoxime; CAZ, ceftazidime; CAZ-CLA, ceftazidime-clavulanic acid; CRO, ceftriaxone; CEF, cephalothin; CIP, ciprofloxacin; GEN, gentamicin; IPM, imipenem; MEM, meropenem; PIP-TZB, piperacillin-tazobactam; NM, not measured.

    • ↵b Bold represents resistance by CLSI interpretation rules.

  • TABLE 2

    Characteristics of β-lactamase and ESBL genes in two E. coli and three Klebsiella pneumoniae subsp. pneumoniae clinical mastitis isolates

    IsolateaESBL/fluoroquinolone genes in the isolatesPresence of replicon type:ST by MLSTPhylogenetic group
    blaCTX-MblaSHVblaTEMblaQnrSB/OP1K/BFIBI1F
    25 (K. pneumoniae)SHV-12TEM-1+−−+−−
    26 (E. coli)CTX-M-15TEM-1−+−−+++ST88A
    27(K. pneumoniae)SHV-12TEM-1+−−+−−−
    28 (K. pneumoniae)SHV-12TEM-1+−−+−−−
    32 (E. coli)CTX-M-15TEM-1−+−−+++A
    26CCTX-M-15TEM-1−+−−+−
    28C−+−−−−
    32CCTX-M-15TEM-1−+−−+−
    K-12−+−−−−
    • ↵a 25, 26, 27, 28, and 32 correspond to 25(2053), 26(2104A), 27(2104B), 28(2104C), and 32(2188), respectively. Isolates 25, 26, 27, 28, and 32 are original isolates; isolates 26C, 28C, and 32C are transconjugants; and K-12 is the E. coli recipient strain.

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Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom
Dorina Timofte, Iuliana E. Maciuca, Nicholas J. Evans, Helen Williams, Andrew Wattret, Jenny C. Fick, Nicola J. Williams
Antimicrobial Agents and Chemotherapy Jan 2014, 58 (2) 789-794; DOI: 10.1128/AAC.00752-13

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Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom
Dorina Timofte, Iuliana E. Maciuca, Nicholas J. Evans, Helen Williams, Andrew Wattret, Jenny C. Fick, Nicola J. Williams
Antimicrobial Agents and Chemotherapy Jan 2014, 58 (2) 789-794; DOI: 10.1128/AAC.00752-13
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