Complete Sequencing of IncI1 Sequence Type 2 Plasmid pJIE512b Indicates Mobilization of bla_CMY-2 from an IncA/C Plasmid

ABSTRACT

Sequencing of pJIE512b, a 92.3-kb IncI1 sequence type 2 (ST2) plasmid carrying bla_CMY-2, revealed a bla_CMY-2 context that appeared to have been mobilized from an IncA/C plasmid by the insertion sequence IS1294. A comparison with published plasmids suggests that bla_CMY-2 has been mobilized from IncA/C to IncI1 plasmids more than once by IS1294-like elements. Alignment of pJIE512b with the only other available IncI1 ST2 plasmid revealed differences across the backbones, indicating variability within this sequence type.