Article Figures & Data
Figures
- FIG 1
Linear comparison of the lsa(E)-carrying plasmid pY13 (GenBank accession no. KR936172) with enterococcal and staphylococcal plasmids pXD4, pXD5, pM7M2, pV7037, and p3. Arrows, positions and directions of transcription of the genes; shading, regions of >99% nucleotide sequence identity in the different plasmids; black arrowheads, the nine overlapping PCRs (PCR1 to PCR9) designed to confirm the pY13 plasmid and the genetic environment of the lsa(E) gene; Δ, a truncated gene.
Tables
- TABLE 1
Antimicrobial susceptibility of the seven lsa(E)-carrying enterococcal isolates, a transformant, and a recipient strain used in this study
Strain MLST type MIC (mg/liter) fora: GEN KAN VIR M1b STR LIN TIA VAL ERY FFC TET CIP AMP RIF VAN LZD E. faecalis 11-27 ST169 128 >256 >128 128 256 128 128 >256 8 64 16 1 8 1 1 E. faecalis 12-7 ST220 >256 >256 >128 256 256 128 128 >256 128 64 32 1 4 1 8 E. faecalis 14-1 ST146 256 >256 >128 256 128 128 128 >256 128 128 64 1 16 2 16 E. faecalis E15 ST283 128 >256 >128 256 128 128 256 >256 128 128 64 2 32 2 16 E. faecalis D12-2 ST553 128 >256 >128 128 128 >128 256 >256 128 64 64 1 2 2 16 E. gallinarum Y15 NAc 256 >256 128 128 128 128 64 >256 128 64 64 0.5 8 2 8 E. faecium Y13 ST29 >256 >256 128 256 128 >128 128 >256 64 64 64 1 2 0.5 16 E. faecalis TY13d 2 >256 128 256 128 >128 128 >256 4 4 1 1 1 ≤0.5 2 E. faecalis JH2-2 2 4 2 2 4 2 2 0.5 4 2 2 1 2 1 2 ↵a GEN, gentamicin; STR, streptomycin; KAN, kanamycin; VIR M1, virginiamycin M1; LIN, lincomycin; TIA, tiamulin; VAL, valnemulin; ERY, erythromycin; FFC, florfenicol; TET, tetracycline; CIP, ciprofloxacin; AMP, ampicillin; RIF, rifampin; VAN, vancomycin; LZD, linezolid.
↵b Note that E. faecalis is intrinsically resistant to streptogramins.
↵c NA, not applicable.
↵d TY13 was the transformant derived from transformation of plasmid pY13 from E. faecium Y13 into E. faecalis JH2-2.
Supplemental material
- Supplemental file 1 -
Tables S1 and S2 and Fig. S1
PDF, 358K
- Supplemental file 1 -
