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Mechanisms of Resistance

Dissemination of the Same cfr-Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China

Jia Chang Cai, Yan Yan Hu, Hong Wei Zhou, Gong-Xiang Chen, Rong Zhang
Jia Chang Cai
The 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, People's Republic of China
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Yan Yan Hu
The 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, People's Republic of China
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Hong Wei Zhou
The 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, People's Republic of China
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Gong-Xiang Chen
The 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, People's Republic of China
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Rong Zhang
The 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, People's Republic of China
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DOI: 10.1128/AAC.04580-14
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    FIG 1

    Genetic map of pLRSA417. Coding regions of >50 amino acids are represented by arrows indicating the direction of transcription, and the corresponding genes are annotated. hp1 to hp12 represent putative genes encoding hypothetical proteins.

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    FIG 2

    Schematic representation and comparison of the cfr genetic environment. Linear genetic maps of plasmids are presented, with the accession numbers given in parentheses. Genes and their corresponding transcription orientations are indicated by arrows with various shading patterns. The region within the two dotted lines between plasmid maps illustrates that they share high homology (>95% nucleotide identity). The structure of a Tn4001-like transposon is indicated. The triangles represent the inverted repeats of the respective mobile elements, and target site duplications are presented as 8-bp sequences.

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  • TABLE 1

    Clinical characteristics of patients with LRSA

    Patient no.IsolateCollection date (mo/yr)SexAge (yr)Days of LRSA isolation/no. of days in ICUAntibiotic therapy within 2 wk prior to LRSA isolationaOutcome
    1LRSA4174/2013Male6616/50TZPSurvived
    2LRSA4224/2013Male653/13NoneDied
    3LRSA5315/2013Male639/26CMN, TZPSurvived
    4LRSA6086/2013Female6122/28MOX, MEM, TECSurvived
    5LRSA6216/2013Male4015/22MEM, TZP, AKSurvived
    6LRSA7267/2013Male4533/58AK, MEM, PBDied
    • ↵a TZP, piperacillin-tazobactam; CMN, cefminox; MOX, moxifloxacin; MEM, meropenem; TEC, teicoplanin; AK, amikacin; PB, polymyxin B.

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      Supplemental Table S1 and Figure S1.

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Dissemination of the Same cfr-Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China
Jia Chang Cai, Yan Yan Hu, Hong Wei Zhou, Gong-Xiang Chen, Rong Zhang
Antimicrobial Agents and Chemotherapy May 2015, 59 (6) 3669-3671; DOI: 10.1128/AAC.04580-14

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Dissemination of the Same cfr-Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China
Jia Chang Cai, Yan Yan Hu, Hong Wei Zhou, Gong-Xiang Chen, Rong Zhang
Antimicrobial Agents and Chemotherapy May 2015, 59 (6) 3669-3671; DOI: 10.1128/AAC.04580-14
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