Article Figures & Data
Figures
- FIG 1
PFGE patterns of 25 blaNDM-positive clinical E. coli isolates.
- FIG 2
S1-PFGE (S1) and Southern hybridization (blaNDM SH) of representative blaNDM-positive E. coli clinical isolates and the corresponding transconjugants. Uppercase Ts denote transconjugants; the strain identification codes are described in Table 1.
- FIG 3
Comparison of PFGE profiles of transconjugants carrying different sizes of plasmids from their parental strains to the PFGE profile of the recipient strain, E. coli EC600.
Tables
- TABLE 1
Profiles of plasmids and β-lactamase genes recoverable in 25 blaNDM-positive E. coli clinical isolates and the corresponding transconjugants
Isolatea MLST β-Lactamase gene(s) in: Estimated size(s) (kb) of plasmid(s) inb: Plasmid type in transconjugantc Parental strain Transconjugant Parental strain Transconjugant SY91 167 blaNDM-5, blaCTX-M-14 blaNDM-5 280, 160, 110, 60 60 IncX3 SY92 167 blaNDM-5, blaCTX-M-14 blaNDM-5 280, 160, 110, 60 60 IncX3 SY93 167 blaNDM-5, blaCTX-M-14 blaNDM-5 280, 135, 60 60 IncX3 JN102 167 blaNDM-5, blaCTX-M-14, blaCTX-M-15, blaTEM-1 blaNDM-5, blaTEM-1 110, 90, 60 60 IncX3 JN105 167 blaNDM-5, blaCTX-M-15, blaTEM-1 blaNDM-5, blaTEM-1 160,135, 100, 60 65 IncX3 JN106 167 blaNDM-5, blaCTX-M-15, blaTEM-1 blaNDM-5, blaTEM-1 160,135, 100, 60 60 IncX3 BJ114 167 blaNDM-5, blaCTX-M-14 blaNDM-5, blaCTX-M-14 160, 100,80, 60 200 IncX3 LZ135 167 blaNDM-5, blaCTX-M-15 blaNDM-5 150, 100 105, 100, 90 IncFrepB JN103 167 blaNDM-1, blaCTX-M-14, blaCTX-M-15, blaTEM-1 blaNDM-1, blaTEM-1 200, 160, 110 160 UT SRM94 167 blaNDM-1, blaTEM-1 blaNDM-1, blaTEM-1 160 160 IncF JX6 167 blaNDM-1, blaCTX-M-14, blaTEM-1, blaSHV-12 blaNDM-1, blaTEM-1, blaSHV-12 110, 100, 60 65 IncX3 JX34 167 blaNDM-1, blaCTX-M-15, blaTEM-1, blaSHV-12 blaNDM-1, blaTEM-1, blaSHV-12 210, 110, 60 60 IncX3 BJ119 405 blaNDM-5, blaCTX-M-15 blaNDM-5 130,70,60 60 IncX3 SRM282 533 blaNDM-5, blaCTX-M-14 blaNDM-5 200, 120, 60 60 IncX3 WH97 410 blaNDM-1, blaCTX-M-14, blaCTX-M-15 blaNDM-1, blaCTX-M-14 100, 75, 60 60 IncN JX45 359 blaNDM-1, blaCTX-M-15, blaTEM-1, blaSHV-12 blaNDM-1, blaTEM-1, blaSHV-12 160, 100, 60 60 IncX3 WH32 1284 blaNDM-1 blaNDM-1 160, 68 230 IncN SY96 2749 blaNDM-1, blaCTX-M-15 blaNDM-1 240, 170, 60 330, 60 IncN SRM251 3489 blaNDM-1, blaSHV-12 blaNDM-1, blaSHV-12 165, 60, 48 60 IncX3 BJ128 5699 blaNDM-1, blaCTX-M-14 blaNDM-1, blaCTX-M-14 200,100,80 200 UT LZ136 167 blaNDM-5, blaCTX-M-15, blaTEM-1 150, 90 NC JX33 167 blaNDM-1, blaCTX-M-15 160, 125, 100 NC BJ116 101 blaNDM-3, blaCTX-M-15 165, 90, 85 NC WH36 1284 blaNDM-1 200 NC SRM49 5138 blaNDM-1, blaCTX-M-14, blaTEM-1 155, 125, 100, 80 NC ↵a Isolate identification codes indicate the source of the isolate as follows: WH, Wuhan, capital city of Hubei Province; SY, Shenyang, capital city of Liaoning Province; JX, Jiaxing, city of Zhejiang Province; LZ, Lanzhou, capital city of Gansu Province; JN, Jinan, capital city of Shandong Province; SMR, People's Hospital of Zhejiang Province in Hangzhou, capital of Zhejiang Province; BJ, Beijing.
↵b Plasmids harboring blaNDM genes are denoted in boldface. NC, nonconjugative.
↵c UT, untypeable.
- TABLE 2
Characteristics of conjugative plasmids carried by the 20 transconjugants
Transconjuganta Result of screening forb: Estimated plasmid size(s) (bp) Plasmid typec β-Lactamase gene(s) repB ISL3-blaSHV-12 bleo-blaNDM-1 blaNDM-1-insH topB-ftsH SY91-T + + + 60 IncX3 blaNDM-5 SY92-T + + + 60 IncX3 blaNDM-5 SY93-T + + + 60 IncX3 blaNDM-5 JN102-T + + + 60 IncX3 blaNDM-5, blaTEM-1 JN105-T + + + 65 IncX3 blaNDM-5, blaTEM-1 JN106-T + + + 60 IncX3 blaNDM-5, blaTEM-1 BJ114-T + + + 200 IncX3 blaNDM-5, blaCTX-M-14 LZ135-T + 105, 100, 90 IncFrepB blaNDM-5 JN103-T + + 160 UT blaNDM-1, blaTEM-1 SRM94-T + 160 IncF blaNDM-1, blaTEM-1 JX6-T + + + + + 65 IncX3 blaNDM-1, blaTEM-1, blaSHV-12 JX34 + + + + + 60 IncX3 blaNDM-1, blaTEM-1, blaSHV-12 BJ119-T + + + 60 IncX3 blaNDM-5 SRM282-T + + + 60 IncX3 blaNDM-5 WH97-T + 60 IncN blaNDM-1, blaCTX-M-14 LX45-T + + + + + 60 IncX3 blaNDM-1, blaTEM-1, blaSHV-12 WH32-T + + 230 IncN blaNDM-1 SY96-T + 330, 60 IncN blaNDM-1 SRM251-T + + + + + 60 IncX3 blaNDM-1, blaSHV-12 BJ128-T + 200 UT blaNDM-1, blaCTX-M-14 ↵a Identification codes are described in Table 1; the uppercase Ts denote transconjugants.
↵b Primers were designed based on the IncX3 plasmid, namely, pNDM-HN380 (NC_019162). Primers targeting repB (positions 54029 to 1127) and topB-ftsH (positions 28908 to 33069) were used to screen for X3-specific conjugative plasmids, the primer targeting ISL3 tnpA-blaSHV-2 (positions 4395 to 8093) was used to screen for the blaSHV-2 gene, the primer targeting bleo-blaNDM-1 (positions 17,418 to 18,100) was used to screen for the conservative blaNDM-1 mobile element, and the primer targeting blaNDM-1-insH (positions 17768 to 20103) was used to screen for the upstream transposase gene.
↵c UT, untypeable.
