ABSTRACT
Lefamulin was evaluated against various Streptococcus pneumoniae serotypes that were collected from adults with lower respiratory tract infections. Lefamulin exhibited MIC50 and MIC90 values of 0.12 and 0.25 μg/ml, respectively, against the entire collection (n = 822). Similar results were obtained for lefamulin against each of the most common serotypes as well as against multidrug-resistant isolates and strains that are nonsusceptible to ceftriaxone or erythromycin. These data support the clinical development of lefamulin for the treatment of community-acquired respiratory tract infections.
TEXT
Community-acquired respiratory tract infections (CARTIs) comprise a series of clinical syndromes, including community-acquired bacterial pneumonia (CABP), bacterial sinusitis, acute otitis media, and acute bacterial exacerbations of chronic bronchitis (1). CARTIs, especially CABP, are among the most frequent infections treated by physicians and represent a major international health problem (2). In addition, CABP represents the leading cause of hospitalizations in the United States and the main cause of morbidity and mortality among children and the elderly, with medical costs estimated at almost $1 billion and $17 billion annually in the United States, respectively (3, 4).
Lefamulin belongs to the pleuromutilin class of antimicrobial agents, which inhibits bacterial protein synthesis by selectively binding to the peptidyl transferase center of the bacterial ribosome and prevents the correct positioning of the 3′-CCA ends of tRNAs for peptide transfer (5–10). Lefamulin is the first pleuromutilin in development for intravenous and oral administration in humans (6), and it is currently in late-stage clinical development for the treatment of CABP and acute bacterial skin and skin structure infections (11–13). Lefamulin exhibits potent antibacterial activity against the most important respiratory and skin pathogens, including Streptococcus spp. and Staphylococcus spp., fastidious Gram-negative organisms, such as Haemophilus influenzae, and atypical respiratory pathogens, including Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila (14–16). In addition, in healthy human subjects, lefamulin (unbound protein) demonstrated extensive penetration and accumulation in pulmonary epithelial lining fluid, reaching a median total area under the concentration-time curve from 0 to 12 h (AUC0–12) of 5.78 μg · h/ml and a median maximum concentration of drug in serum (Cmax) of ∼0.7 μg/ml after a single 150-mg intravenous infusion over 1 h (17). This study evaluated the in vitro activity of lefamulin against specific serotypes of clinical isolates of Streptococcus pneumoniae.
A total of 822 S. pneumoniae isolates from 58 hospitals located in the nine United States Census regions as part of the SENTRY Antimicrobial Surveillance Program for 2010 were included. Isolates were recovered from lower respiratory tract specimens of adult patients aged ≥18 years and were submitted to a central monitoring laboratory (JMI Laboratories, North Liberty, IA, USA) for confirmation of bacterial identification, which was performed by biochemical algorithms and/or PCR assays as previously described (18). Serotypes were determined according to the sequence of cpsB in combination with multiplex PCR assays and the capsular swelling method (18). Isolates were tested for susceptibility by broth microdilution (19). Testing was performed using dry-form panels manufactured by Thermo Fisher Scientific (Cleveland, OH, USA) under appropriate quality assurance (20). Susceptibility interpretive criteria for comparator agents were those established by the Clinical and Laboratory Standards Institute and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) (20, 21). An isolate was categorized as multidrug resistant (MDR) if it had elevated MIC results for three or more of the following drug classes (drug class probe): penicillins (penicillin, ≥4 μg/ml), cephalosporins (ceftriaxone, ≥2 μg/ml), macrolides (erythromycin, ≥0.5 μg/ml), tetracyclines (tetracycline HCl, ≥2 μg/ml), fluoroquinolones (levofloxacin, ≥4 μg/ml), lincosamides (clindamycin, ≥0.5 μg/ml), or folate pathway inhibitors (trimethoprim-sulfamethoxazole [TMP-SMX], ≥1/19 μg/ml).
In general, lefamulin exhibited a log-normal MIC distribution against the population of S. pneumoniae, with modal MIC, MIC50, and MIC90 results of 0.12, 0.12, and 0.25 μg/ml, respectively. In addition, all isolates were inhibited by lefamulin at ≤1 μg/ml (Table 1). Overall, MIC50 and MIC90 results for lefamulin remained at 0.12 and 0.25 μg/ml, respectively, when tested against (i) the most common serotypes, (ii) isolates displaying a nonsusceptible phenotype to ceftriaxone or erythromycin, and (iii) the MDR subset (Table 1). Slightly different MIC50 and MIC90 values were observed for lefamulin when it was tested against serotypes 3 and 11A/11D, showing lefamulin MIC50 and MIC90 results of 0.06 and 0.12 μg/ml and 0.25 and 0.5 μg/ml, respectively.
Lefamulin in vitro activity and cumulative MIC distributions against the overall population of S. pneumoniae selected serotypes and resistance subsets
The MIC values observed for lefamulin (MIC50 and MIC90, 0.12 and 0.25 μg/ml, respectively) against all isolates were similar to those obtained for imipenem (MIC50 and MIC90, ≤0.12 and 0.25 μg/ml; 100.0% susceptible) and were 2- to 4-fold lower than those for vancomycin (MIC50 and MIC90, 0.25 and 0.5 μg/ml, respectively; 100.0% susceptible), linezolid (MIC50 and MIC90, 1 and 1 μg/ml, respectively; 99.9% susceptible; data not shown), and levofloxacin (MIC50 and MIC90, 1 and 1 μg/ml, respectively; 98.9% susceptible) (Tables 2 and 3). Other comparators showed decreased antimicrobial activity against all S. pneumoniae isolates, including ceftriaxone (MIC50 and MIC90, ≤0.06 and 1 μg/ml, respectively; 92.6% susceptible), penicillin (MIC50 and MIC90, ≤0.03 and 4 μg/ml, respectively; 89.2% susceptible), clindamycin (MIC50 and MIC90, ≤0.25 and >1 μg/ml, respectively; 81.6% susceptible), erythromycin (MIC50 and MIC90, ≤0.06 and >8 μg/ml, respectively; 62.9% susceptible), tetracycline (MIC50 and MIC90, 0.5 and >8 μg/ml, respectively; 79.7% susceptible), and TMP-SMX (MIC50 and MIC90, ≤0.5 and 4 μg/ml, respectively; 71.3% susceptible) (Tables 2 and 3).
Antimicrobial susceptibility of the overall population of S. pneumoniae selected serotypes and resistance subsets based on CLSI breakpoints
In vitro activity of lefamulin and comparator agents against the overall population of S. pneumoniae selected serotypes and resistance subsets
Isolates were highly susceptible to penicillin and ceftriaxone except for serotypes 19A and 19F, for which susceptibility ranged from 38.2% to 75.0% (Table 2). When EUCAST breakpoints were applied, the proportions of intermediate and resistant isolates increased for the two compounds, especially for serotypes 19A, 35B, 6C/6D, 15A, and 19F (see Table S1 in the supplemental material). Clindamycin was also active against most serotypes, excluding 19A, 19F, and 15A/15F. In contrast, erythromycin had limited activity, with only serotypes 3 and 7F demonstrating high susceptibility rates (92.9% to 95.6% susceptible). Susceptibility rates obtained for tetracycline and TMP-SMX against most common serotypes varied, with the highest resistance rates among the serotypes 19F and 15A/15F for tetracycline and 19A, 6C/6D, and 19F for TMP-SMX; the proportion of intermediate isolates increased using EUCAST breakpoints.
A total of 21.9% of tested isolates displayed a MDR phenotype, and the majority (51.1%) of these S. pneumoniae isolates were serotype 19A or 19F, while 42.8% or 35.6% of MDR isolates are not covered by the 13-valent pneumococcal conjugate vaccine (PCV13) or the 23-valent pneumococcal polysaccharide vaccine (PPSV23), respectively (Table 1). Only lefamulin (MIC50 and MIC90, 0.12 and 0.25 μg/ml, respectively) (Tables 1 and 3), vancomycin (MIC50 and MIC90, 0.25 and 0.5 μg/ml, respectively), linezolid (MIC50 and MIC90, 1 and 1 μg/ml, respectively), and levofloxacin (MIC50 and MIC90, 1 and 1 μg/ml, respectively) demonstrated in vitro activity against MDR isolates (data not shown).
Recent reports have indicated a decrease in the prevalence of serotypes associated with PCV13 in children and adults (18, 22, 23), while other studies have documented an increase in non-PCV13 serotypes, including those with decreased antimicrobial susceptibility (24–27). Under these scenarios, additional alternatives for the treatment of CABP are needed. Potent and consistent lefamulin activity was observed regardless of serotype, including those not covered by PCV13 and those with a nonsusceptible phenotype to ceftriaxone or erythromycin or a MDR phenotype. The reasons for the small variation of lefamulin activity within serotypes (i.e., 3 and 11A/11D) need further investigation and may have been due to sample size.
Overall, when viewed in the context of the high pulmonary penetration observed in human subjects, these in vitro data support the continued clinical development of lefamulin for the treatment of patients with CABP, including those clinical cases caused by less-susceptible isolates.
ACKNOWLEDGMENTS
We thank the following staff members at JMI Laboratories (North Liberty, IA, USA) for technical support and/or assistance with manuscript preparation: M. Castanheira, L. Duncan, M. Janechek, R. Jones, M. Huband, J. Oberholser, P. Rhomberg, J. Ross, J. Schuchert, J. Streit, and L. Woosley.
JMI Laboratories, Inc. has received research and educational grants from 2014 to 2015 from Achaogen, Actavis, Actelion, Allergan, American Proficiency Institute (API), AmpliPhi, Anacor, Astellas, AstraZeneca, Basilea, Bayer, BD, Cardeas, Cellceutix, CEM-102 Pharmaceuticals, Cempra, Cerexa, Cidara, CorMedix, Cubist, Debiopharm, Dipexium, Dong Wha, Durata, Enteris, Exela, Forest Research Institute, Furiex, Genentech, GSK, Helperby, ICPD, Janssen, Lannett, Longitude, Medpace, Meiji Seika Kasha, Melinta, Merck, Motif, Nabriva, Novartis, Paratek, Pfizer, Pocared, PTC Therapeutics, Rempex, Roche, Salvat, Scynexis, Seachaid, Shionogi, Tetraphase, The Medicines Co., Theravance, Thermo Fisher, VenatoRx, Vertex, Wockhardt, and Zavante. Some JMI employees are advisors/consultants for Allergan, Astellas, Cubist, Pfizer, Cempra, and Theravance. With regard to speakers' bureaus and stock options, we have no conflicts of interest to declare.
FOOTNOTES
- Received 18 March 2016.
- Returned for modification 7 April 2016.
- Accepted 1 May 2016.
- Accepted manuscript posted online 9 May 2016.
Supplemental material for this article may be found at http://dx.doi.org/10.1128/AAC.00627-16.
- Copyright © 2016, American Society for Microbiology. All Rights Reserved.