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Epidemiology and Surveillance

Detection of the mcr-1 Colistin Resistance Gene in Carbapenem-Resistant Enterobacteriaceae from Different Hospitals in China

Hua Yu, Fen Qu, Bin Shan, Bin Huang, Wei Jia, Cha Chen, Aiqing Li, Minhui Miao, Xin Zhang, Chunmei Bao, Yunmin Xu, Kalyan D. Chavda, Yi-Wei Tang, Barry N. Kreiswirth, Hong Du, Liang Chen
Hua Yu
aSichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China
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Fen Qu
bThe Center of Clinical Diagnosis Laboratory, 302 Hospital of PLA, Beijing, China
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Bin Shan
cDepartment of Laboratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
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Bin Huang
dDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China
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Wei Jia
eCenter of Medical Laboratory, the General Hospital of Ningxia Medical University, Yinchuan, China
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Cha Chen
fDepartment of Laboratory Medicine, The Traditional Chinese Medical Hospital of Guangdong, Guangzhou, Guangdong, China
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Aiqing Li
gDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
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Minhui Miao
gDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
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Xin Zhang
aSichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China
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Chunmei Bao
bThe Center of Clinical Diagnosis Laboratory, 302 Hospital of PLA, Beijing, China
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Yunmin Xu
cDepartment of Laboratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
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Kalyan D. Chavda
hPublic Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA
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Yi-Wei Tang
iDepartment of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA
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  • ORCID record for Yi-Wei Tang
Barry N. Kreiswirth
hPublic Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA
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Hong Du
gDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
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Liang Chen
hPublic Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA
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DOI: 10.1128/AAC.00440-16
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    FIG 1

    Chromosomal integration of mcr-1 gene. Arrows denote open reading frames (ORF), with red, gray, yellow, and white arrows denoting mcr-1, pap2, ISApl1, and neighboring chromosomal genes (illustrated by locus_tag in ST156 strain SE11 [GenBank accession no. AP009240]), respectively. The small black arrows adjacent to ISApl1 denote the inverted reverse repeats (IRR). The 2-bp putative target duplicated sequences for TnApl1 are underlined.

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  • TABLE 1

    Characteristics of MCR-1-producing E. coli strains and their E. coli J53 transconjugantsa

    E. coli strainSTβ-Lactamase(s)PlasmidMIC (μg/ml)b
    SAMAMCCFZCAZCTXFEPATMIMPMEMAMKGENCIPLVXMXFTZPSXTTETCHLCOL
    CDA6167NDM-5, CMY-2, TEM-1ND>16/8>16/8>16>16>32>16≤2>8>8≤8<1>2>8>4>64/4>2/38≤28>2
    BJ10156NDM-5, TEM-1ND>16/8>16/8>16>16>32>16>16>8>8≤8>8>2>8>4>64/4>2/38>8>16>2
    BJ13457CTX-M-14, CMY-2, TEM-1ND>16/8>16/8>16>16>32>1616>84≤8>8>2>8>4>64/4≤0.5/9>88>2
    CDA6-mcr-TND—IncX4≤4/2≤8/4≤4≤1≤1≤2≤2≤1≤1≤8≤2≤0.5≤1≤1≤4/2≤0.5/9≤2≤4>2
    BJ13-mcr-TND—IncI2≤4/2≤8/4≤4≤1≤1≤2≤2≤1≤1≤8≤2≤0.5≤1≤1≤4/2≤0.5/9≤2≤4>2
    CDA6-NDM-TNDNDM-5IncX3>16/8>16/8>16>16>32>16≤2>8>8≤8≤2≤0.5≤1≤1>64/4≤0.5/9≤2≤4≤0.5
    BJ10-NDM-TNDNDM-5IncX3>16/8>16/8>16>16>32>16≤2>8>8≤8≤2≤0.5≤1≤164/4≤0.5/9≤2≤4≤0.5
    • ↵a CDA6-mcr-T and BJ13-mcr-T, mcr-1 E. coli J53 transconjugants; CDA6-NDM-T and BJ10-NDM-T, blaNDM-5 E. coli J53 transconjugants. No mcr-1 transconjugant was obtained from BJ10. ND, not determined; —, negative by PCR.

    • ↵b MICs were determined using a MicroScan WalkAway Plus system. SAM, ampicillin-sulbactam; AMC, amoxicillin-clavulanic acid; CFZ, cefazolin; CAZ, ceftazidime; CTX, cefotaxime; FEP, cefepime; ATM, aztreonam; IMP, imipenem; MEM, meropenem; AMK, amikacin, GEN, gentamicin; CIP, ciprofloxacin; LVX, levofloxacin; MXF, moxifloxacin; TZP, piperacillin-tazobactam; SXT, trimethoprim-sulfamethoxazole; TET, tetracycline; CHL, Chloramphenicol; COL, colistin.

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Detection of the mcr-1 Colistin Resistance Gene in Carbapenem-Resistant Enterobacteriaceae from Different Hospitals in China
Hua Yu, Fen Qu, Bin Shan, Bin Huang, Wei Jia, Cha Chen, Aiqing Li, Minhui Miao, Xin Zhang, Chunmei Bao, Yunmin Xu, Kalyan D. Chavda, Yi-Wei Tang, Barry N. Kreiswirth, Hong Du, Liang Chen
Antimicrobial Agents and Chemotherapy Jul 2016, 60 (8) 5033-5035; DOI: 10.1128/AAC.00440-16

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Detection of the mcr-1 Colistin Resistance Gene in Carbapenem-Resistant Enterobacteriaceae from Different Hospitals in China
Hua Yu, Fen Qu, Bin Shan, Bin Huang, Wei Jia, Cha Chen, Aiqing Li, Minhui Miao, Xin Zhang, Chunmei Bao, Yunmin Xu, Kalyan D. Chavda, Yi-Wei Tang, Barry N. Kreiswirth, Hong Du, Liang Chen
Antimicrobial Agents and Chemotherapy Jul 2016, 60 (8) 5033-5035; DOI: 10.1128/AAC.00440-16
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