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Mechanisms of Resistance

Community Composition Determines Activity of Antibiotics against Multispecies Biofilms

Sarah Tavernier, Aurélie Crabbé, Mayram Hacioglu, Liesbeth Stuer, Silke Henry, Petra Rigole, Inne Dhondt, Tom Coenye
Sarah Tavernier
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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Aurélie Crabbé
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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Mayram Hacioglu
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
bLaboratory of Pharmaceutical Microbiology, Istanbul University, Istanbul, Turkey
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Liesbeth Stuer
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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Silke Henry
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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Petra Rigole
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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Inne Dhondt
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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Tom Coenye
aLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
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  • ORCID record for Tom Coenye
DOI: 10.1128/AAC.00302-17
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ABSTRACT

In young cystic fibrosis (CF) patients, Staphylococcus aureus is typically the most prevalent organism, while in adults, Pseudomonas aeruginosa is the major pathogen. More recently, it was observed that also Streptococcus anginosus plays an important role in exacerbations of respiratory symptoms. These species are often coisolated from CF lungs, yet little is known about whether antibiotic killing of one species is influenced by the presence of others. In the present study, we compared the activities of various antibiotics against S. anginosus, S. aureus, and P. aeruginosa when grown in monospecies biofilms with the activity observed in a multispecies biofilm. Our results show that differences in antibiotic activity against species grown in mono- and multispecies biofilms are species and antibiotic dependent. Fewer S. anginosus cells are killed by antibiotics that interfere with cell wall synthesis (amoxicillin plus sulbactam, cefepime, imipenem, meropenem, and vancomycin) in the presence of S. aureus and P. aeruginosa, while for ciprofloxacin, levofloxacin, and tobramycin, no difference was observed. In addition, we observed that the cell-free supernatant of S. aureus, but not that of P. aeruginosa biofilms, also caused this decrease in killing. Overall, S. aureus was more affected by antibiotic treatment in a multispecies biofilm, while for P. aeruginosa, no differences were observed between growth in mono- or multispecies biofilms. The results of the present study suggest that it is important to take the community composition into account when evaluating the effect of antimicrobial treatments against certain species in mixed biofilms.

FOOTNOTES

    • Received 9 February 2017.
    • Returned for modification 8 March 2017.
    • Accepted 2 July 2017.
    • Accepted manuscript posted online 10 July 2017.
  • Supplemental material for this article may be found at https://doi.org/10.1128/AAC.00302-17 .

  • Copyright © 2017 American Society for Microbiology.

All Rights Reserved .

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Community Composition Determines Activity of Antibiotics against Multispecies Biofilms
Sarah Tavernier, Aurélie Crabbé, Mayram Hacioglu, Liesbeth Stuer, Silke Henry, Petra Rigole, Inne Dhondt, Tom Coenye
Antimicrobial Agents and Chemotherapy Aug 2017, 61 (9) e00302-17; DOI: 10.1128/AAC.00302-17

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Community Composition Determines Activity of Antibiotics against Multispecies Biofilms
Sarah Tavernier, Aurélie Crabbé, Mayram Hacioglu, Liesbeth Stuer, Silke Henry, Petra Rigole, Inne Dhondt, Tom Coenye
Antimicrobial Agents and Chemotherapy Aug 2017, 61 (9) e00302-17; DOI: 10.1128/AAC.00302-17
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KEYWORDS

Anti-Bacterial Agents
biofilms
Pseudomonas aeruginosa
Staphylococcus aureus
Streptococcus anginosus
multispecies biofilm
Pseudomonas aeruginosa
Staphylococcus aureus
Streptococcus anginosus

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