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Antiviral Agents

Simple In Vitro Assay To Evaluate the Incorporation Efficiency of Ribonucleotide Analog 5′-Triphosphates into RNA by Human Mitochondrial DNA-Dependent RNA Polymerase

Gaofei Lu, Gregory R. Bluemling, Shuli Mao, Michael Hager, Bharat P. Gurale, Paul Collop, Damien Kuiper, Kasinath Sana, George R. Painter, Abel De La Rosa, Alexander A. Kolykhalov
Gaofei Lu
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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Gregory R. Bluemling
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
bDrug Innovation Ventures at Emory (DRIVE), Atlanta, Georgia, USA
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Shuli Mao
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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Michael Hager
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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Bharat P. Gurale
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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Paul Collop
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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Damien Kuiper
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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Kasinath Sana
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
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George R. Painter
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
bDrug Innovation Ventures at Emory (DRIVE), Atlanta, Georgia, USA
cDepartment of Pharmacology, Emory University School of Medicine, Atlanta, Georgia, USA
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Abel De La Rosa
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
bDrug Innovation Ventures at Emory (DRIVE), Atlanta, Georgia, USA
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Alexander A. Kolykhalov
aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
bDrug Innovation Ventures at Emory (DRIVE), Atlanta, Georgia, USA
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DOI: 10.1128/AAC.01830-17
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Article Information

DOI 
https://doi.org/10.1128/AAC.01830-17
PubMed 
29180528

Published By 
American Society for Microbiology Journals
History 
  • Received September 6, 2017
  • Returned for modification October 4, 2017
  • Accepted November 21, 2017
  • Published online January 25, 2018.

Copyright & Usage 
Copyright © 2018 Lu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Article Versions

  • Accepted Manuscript version (November 27, 2017).
  • You are viewing the most recent version of this article.

Author Information

  1. Gaofei Lua*,
  2. Gregory R. Bluemlinga,b,
  3. Shuli Maoa,
  4. Michael Hagera,
  5. Bharat P. Guralea,
  6. Paul Collopa,
  7. Damien Kuipera,
  8. Kasinath Sanaa*,
  9. George R. Paintera,b,c,
  10. Abel De La Rosaa,b and
  11. Alexander A. Kolykhalova,b
  1. aEmory Institute for Drug Development (EIDD), Emory University, Atlanta, Georgia, USA
  2. bDrug Innovation Ventures at Emory (DRIVE), Atlanta, Georgia, USA
  3. cDepartment of Pharmacology, Emory University School of Medicine, Atlanta, Georgia, USA
  • Address correspondence to Alexander A. Kolykhalov, a.kolykhalov{at}emory.edu.
  • ↵* Present address: Gaofei Lu, Global Health Drug Discovery Institute (GHDDI), Beijing, China; Kasinath Sana, E. I. DuPont India Pvt. Ltd., Dupont Knowledge Center, Hyderabad, India.

  • Citation Lu G, Bluemling GR, Mao S, Hager M, Gurale BP, Collop P, Kuiper D, Sana K, Painter GR, De La Rosa A, Kolykhalov AA. 2018. Simple in vitro assay to evaluate the incorporation efficiency of ribonucleotide analog 5′-triphosphates into RNA by human mitochondrial DNA-dependent RNA polymerase. Antimicrob Agents Chemother 62:e01830-17. https://doi.org/10.1128/AAC.01830-17.

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Simple In Vitro Assay To Evaluate the Incorporation Efficiency of Ribonucleotide Analog 5′-Triphosphates into RNA by Human Mitochondrial DNA-Dependent RNA Polymerase
Gaofei Lu, Gregory R. Bluemling, Shuli Mao, Michael Hager, Bharat P. Gurale, Paul Collop, Damien Kuiper, Kasinath Sana, George R. Painter, Abel De La Rosa, Alexander A. Kolykhalov
Antimicrobial Agents and Chemotherapy Jan 2018, 62 (2) e01830-17; DOI: 10.1128/AAC.01830-17

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Simple In Vitro Assay To Evaluate the Incorporation Efficiency of Ribonucleotide Analog 5′-Triphosphates into RNA by Human Mitochondrial DNA-Dependent RNA Polymerase
Gaofei Lu, Gregory R. Bluemling, Shuli Mao, Michael Hager, Bharat P. Gurale, Paul Collop, Damien Kuiper, Kasinath Sana, George R. Painter, Abel De La Rosa, Alexander A. Kolykhalov
Antimicrobial Agents and Chemotherapy Jan 2018, 62 (2) e01830-17; DOI: 10.1128/AAC.01830-17
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KEYWORDS

discrimination value
POLRMT
RNA-dependent RNA polymerase
RdRp
human mitochondrial RNA polymerase
mitochondrial polymerase
nonradioactive assay
nucleoside analogs
primer extension
ribonucleotide

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