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Mechanisms of Resistance

Detection of Candida auris Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs

Milena Kordalewska, Annie Lee, Yanan Zhao, David S. Perlin
Milena Kordalewska
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA
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Annie Lee
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA
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Yanan Zhao
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA
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David S. Perlin
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA
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DOI: 10.1128/AAC.01754-19
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ABSTRACT

Accurate and rapid assessment of Candida auris antifungal drug resistance is crucial for effective infection prevention and control actions, as well as for patient management. Here, performance of a molecular diagnostic platform, enabling rapid identification of FKS1 and ERG11 mutations conferring echinocandin and azole resistance, respectively, was evaluated on a panel of clinical skin swabs. Gene sequencing and antifungal susceptibility testing were used as the gold standard. All swabs were correctly categorized as harboring wild-type or mutant C. auris.

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Detection of Candida auris Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs
Milena Kordalewska, Annie Lee, Yanan Zhao, David S. Perlin
Antimicrobial Agents and Chemotherapy Nov 2019, 63 (12) e01754-19; DOI: 10.1128/AAC.01754-19

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Detection of Candida auris Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs
Milena Kordalewska, Annie Lee, Yanan Zhao, David S. Perlin
Antimicrobial Agents and Chemotherapy Nov 2019, 63 (12) e01754-19; DOI: 10.1128/AAC.01754-19
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KEYWORDS

Candida
Candida auris
antifungal resistance
antifungal susceptibility testing
azoles
diagnostics
drug resistance mechanisms
echinocandins

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