Article Figures & Data
Figures
- FIG 1
Comparison of daptomycin activity with and without CRO in various models in S. mitis/oralis 351. There was an 18-h delay in reaching the detection limit for DAP 6 mg/kg-CRO 0.5 g (single dose) in comparison to that for DAP 6 mg/kg-CRO 2 g.
- FIG 2
Comparison of daptomycin activity with and without CRO in various models in S. mitis/oralis SF100. DAP 6 mg/kg-CRO 0.5 g (single dose) and DAP 6 mg/kg-CRO 2 g reached the detection limit at the same time, but slight regrowth was observed in DAP 6 mg/kg-CRO 0.5 g (single dose) after 48 h.
- FIG 3
Phospholipid spots shown in 2D-TLC plates were developed with CuSO4 (100 mg/ml) spray containing 8% phosphoric acid and heated at 180°C. PG, phosphatidylglycerol; CL, cardiolipin; PA, phosphatidic acid; UI, unidentified; phospholipid, glycolipid contaminant seen in all S. mitis/oralis strains (previously detailed [29]).
Tables
- TABLE 2
PK data for different doses in SEV model
Dose Cmax (mg/liter) AUC0–24 (mg · h/liter) t1/2 (h) DAP 6 mg/kg 93.27 ± 0.51 1,029.19 ± 43.73 7.89 ± 0.19 h DAP 8 mg/kg 124.82 ± 0.72 1,404.865 ± 7.82 7.93 ± 0.03 DAP 10 mg/kg 143.98 ± 0.35 1,759.41 ± 59.90 8.21 ± 0.11 DAP 12 mg/kg 186 ± 3.32 2,251.99 ± 80.31 7.98 ± 0.08 CRO 2 g/kg 278.9 ± 8.41 3,346.35 ± 108.52 7.88 ± 0.01 CRO 0.5 g/kg 66.75 ± 2.41 756.27 ± 7.45 7.86 ± 0.08 - TABLE 3
Relative surface charge and CM fluidity of study strains
Strain pairsa Surface charge (%)b CM fluidity (PI value) 351/D12T96 A1 59 ± 9/30 ± 4c 0.31 ± 0.02/0.29 ± 0.03c SF100/D12T96 B2 24 ± 4/25 ± 5 0.29 ± 0.00/0.30 ± 0.01 ↵a First strain in each pair is the DAP-S parental strain, and the second one is the DAP-R strain.
↵b Percentage of bacterial-bound Cyt C calculated from the proportion of unbound Cyt C remaining in the supernatant.
↵c P value of <0.05 in 351 DAP-R (D12T96 A1) versus 351 DAP-S S. mitis/oralis parental strain.
