Natamycin and Voriconazole Exhibit Synergistic Interactions with Nonantifungal Ophthalmic Agents against Fusarium Species Ocular Isolates

DOI: 10.1128/AAC.02505-18

Article Figures & Data

TABLE 1

MIC results for Fusarium species ocular isolates^a

↵a NAT, natamycin; VOR, voriconazole; 5FU, 5-fluorouracil; DRZ, dorzolamide; TIM, timolol.

TABLE 2

FICI results for Fusarium species ocular isolates

↵a 5FU, 5-fluorouracil; DRZ, dorzolamide; TIM, timolol. Bold values indicate that the FICI is in the synergistic range (<0.5).

TABLE 3

MICs for the synergistically interacting antifungal and nonantifungal ophthalmic agents natamycin and timolol

Strain	MIC (μg/ml) of each agent alone		MIC (μg/ml) of each agent in the combination		FICI	Avg FICI
Strain	Natamycin	Timolol	Natamycin	Timolol	FICI	Avg FICI
F. solani
132.02	16	>6,800	8	425	0.53	0.52
			4	3,400	0.50
06-0487	32	>6,800	16	213	0.52	0.45
			8	1,700	0.38
06-0330	32	>6,800	16	213	0.50	0.41
			8	850	0.31
06-0133	32	>6,800	16	53	0.50	0.41
			8	850	0.31
06-0111	32	>6,800	16	213	0.52	0.52
06-0110	32	>6,800	16	53	0.50	0.41
			8	850	0.31
F. oxysporum
06-0342	32	>6,800	16	213	0.52	0.41
			8	850	0.31
06-0197	16	>6,800	8	53	0.50	0.44
			4	1,700	0.38

TABLE 4

MICs for the synergistically interacting antifungal and nonantifungal ophthalmic agents natamycin and 5-fluorouracil

Strain	MIC (μg/ml) of each agent alone		MIC (μg/ml) of each agent in the combination		FICI	Avg FICI
Strain	Natamycin	5-Fluorouracil	Natamycin	5-Fluorouracil	FICI	Avg FICI
F. solani
132.02	16	1,250	8	78	0.56	0.59
			2	625	0.63
06-0487	16	2,500	8	156	0.56	0.56
			4	625	0.50
			2	1,250	0.63
06-0330	32	2,500	16	10	0.50	0.40
			8	156	0.31
			4	625	0.38
06-0133	32	2,500	16	10	0.50	0.34
			8	78	0.28
			4	313	0.25
			2	625	0.31
06-0111	32	2,500	16	10	0.50	0.44
			8	156	0.31
			4	625	0.38
			2	1,250	0.56
06-0110	32	2,500	16	10	0.50	0.40
			8	156	0.31
			4	625	0.38
F. oxysporum
06-0342	32	5,000	16	78	0.52	0.40
			8	313	0.31
			4	1,250	0.38
06-0197	16	5,000	8	156	0.53	0.55
			4	1,250	0.50
			2	2,500	0.63