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Susceptibility

Natamycin and Voriconazole Exhibit Synergistic Interactions with Nonantifungal Ophthalmic Agents against Fusarium Species Ocular Isolates

Christiaan A. Rees, Ruina Bao, Michael E. Zegans, Robert A. Cramer
Christiaan A. Rees
aDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
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Ruina Bao
aDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
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Michael E. Zegans
aDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
bDepartment of Surgery, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA
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Robert A. Cramer
aDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
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DOI: 10.1128/AAC.02505-18
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Tables

  • TABLE 1

    MIC results for Fusarium species ocular isolatesa

    IsolateAntifungal MIC (μg/ml)Nonantifungal MIC (μg/ml)
    NATVOR5FUDRZEDTATIM
    F. solani
        132.0216161,880>5,575403>6,800
        06-048726>1283,750>5,575819>6,800
        06-033026>1282,500>5,5752,041>6,800
        06-013329>1282,500>5,575819>6,800
        06-011132>1282,500>5,5751,222>6,800
        06-011029>1282,500>5,5752,041>6,800
    F. oxysporum
        06-034226>1285,000>5,5752,041>6,800
        06-01971443,750>5,5751,222>6,800
    • ↵a NAT, natamycin; VOR, voriconazole; 5FU, 5-fluorouracil; DRZ, dorzolamide; TIM, timolol.

  • TABLE 2

    FICI results for Fusarium species ocular isolates

    IsolateFICIa
    Natamycin plus:Voriconazole plus:
    5FUDRZEDTATIM5FUDRZEDTATIM
    F. solani
        132.020.610.751.000.520.722.001.000.94
        06-04870.560.750.670.450.752.000.380.53
        06-03300.400.640.660.410.592.000.630.69
        06-01330.340.690.750.410.532.000.520.58
        06-01110.441.000.380.520.592.000.530.66
        06-01100.400.530.470.410.692.000.520.66
    F. oxysporum
        06-03420.400.630.520.410.632.000.450.39
        06-01970.550.530.560.440.582.000.550.94
    • ↵a 5FU, 5-fluorouracil; DRZ, dorzolamide; TIM, timolol. Bold values indicate that the FICI is in the synergistic range (<0.5).

  • TABLE 3

    MICs for the synergistically interacting antifungal and nonantifungal ophthalmic agents natamycin and timolol

    StrainMIC (μg/ml) of each agent aloneMIC (μg/ml) of each agent in the combinationFICIAvg FICI
    NatamycinTimololNatamycinTimolol
    F. solani
        132.0216>6,80084250.530.52
        43,4000.50
        06-048732>6,800162130.520.45
        81,7000.38
        06-033032>6,800162130.500.41
        88500.31
        06-013332>6,80016530.500.41
        88500.31
        06-011132>6,800162130.520.52
        06-011032>6,80016530.500.41
    88500.31
    F. oxysporum
        06-034232>6,800162130.520.41
        88500.31
        06-019716>6,8008530.500.44
    41,7000.38
  • TABLE 4

    MICs for the synergistically interacting antifungal and nonantifungal ophthalmic agents natamycin and 5-fluorouracil

    StrainMIC (μg/ml) of each agent aloneMIC (μg/ml) of each agent in the combinationFICIAvg FICI
    Natamycin5-FluorouracilNatamycin5-Fluorouracil
    F. solani
        132.02161,2508780.560.59
        26250.63
        06-0487162,50081560.560.56
        46250.50
        21,2500.63
        06-0330322,50016100.500.40
        81560.31
        46250.38
        06-0133322,50016100.500.34
        8780.28
        43130.25
        26250.31
        06-0111322,50016100.500.44
        81560.31
        46250.38
        21,2500.56
        06-0110322,50016100.500.40
        81560.31
        46250.38
    F. oxysporum
        06-0342325,00016780.520.40
        83130.31
        41,2500.38
        06-0197165,00081560.530.55
    41,2500.50
    22,5000.63
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Natamycin and Voriconazole Exhibit Synergistic Interactions with Nonantifungal Ophthalmic Agents against Fusarium Species Ocular Isolates
Christiaan A. Rees, Ruina Bao, Michael E. Zegans, Robert A. Cramer
Antimicrobial Agents and Chemotherapy Jun 2019, 63 (7) e02505-18; DOI: 10.1128/AAC.02505-18

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Natamycin and Voriconazole Exhibit Synergistic Interactions with Nonantifungal Ophthalmic Agents against Fusarium Species Ocular Isolates
Christiaan A. Rees, Ruina Bao, Michael E. Zegans, Robert A. Cramer
Antimicrobial Agents and Chemotherapy Jun 2019, 63 (7) e02505-18; DOI: 10.1128/AAC.02505-18
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KEYWORDS

Fusarium
antifungal susceptibility testing
fungal keratitis
ocular

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