DOI: 10.1128/AAC.00224-20
ABSTRACT
We isolated spontaneous levofloxacin-resistant strains of Mycobacterium aurum to study the fitness cost and compensatory evolution of fluoroquinolone resistance in mycobacteria. Five of six mutant strains with substantial growth defects showed restored fitness after being serially passaged for 18 growth cycles, along with increased cellular ATP level. Whole-genome sequencing identified putative compensatory mutations in the glgC gene that restored the fitness of the resistant strains, presumably by altering the bacterial energy metabolism.
FOOTNOTES
- Received 5 February 2020.
- Returned for modification 5 March 2020.
- Accepted 25 May 2020.
- Accepted manuscript posted online 1 June 2020.
Supplemental material is available online only.
- Copyright © 2020 American Society for Microbiology.
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