Clinical Therapeutics

A Multimethod, Multicountry Evaluation of Breakpoints for Bedaquiline Resistance Determination

Nazir Ahmed Ismail, Akio Aono, Emanuele Borroni, Daniela Maria Cirillo, Christel Desmaretz, Rumina Hasan, Satoshi Mitarai, Sadia Shakoor, Gabriela Torrea, Koné Kaniga, Shaheed V. Omar
DOI: 10.1128/AAC.00479-20

Article Figures & Data

Figures

  • FIG 1
    FIG 1

    BDQ MIC distribution for BMD frozen plates by particular laboratory (a) and by all laboratories (n = 494) (b). The total number of isolates was 494 (Lab 1, 104; Lab 2, 100; Lab 3, 100; Lab 4, 91; and Lab 5, 99). The arrow indicates the ECOFF determined by visual inspection.

  • FIG 2
    FIG 2

    BDQ MIC distribution for BMD dry plates by particular laboratory (a) and by all laboratories (n = 494) (b). The total number of isolates was 494 (Lab 1, 104; Lab 2, 100; Lab 3, 100; Lab 4, 91; and Lab 5, 99). The arrow indicates the ECOFF determined by visual inspection.

  • FIG 3
    FIG 3

    ECOFF plots and values for BMD frozen (a) and dry (b) plates.

  • FIG 4
    FIG 4

    Diagrammatic representation of BDQ DST results for discordance isolates by method stratified by CRS susceptible (n = 14) (a) and resistant (n = 10) (b). AP, agar proportion; BMD, broth microdilution.

Tables

  • TABLE 1

    Rv0678 variants and number of DST methods classifying resistance (n = 28)a

    TABLE 1

    • ↵a RAV, resistance-associated variant; WT, wild-type (includes the –11C>A mutation in one isolate).

  • TABLE 2

    Prevalence of BDQ resistance by method and composite reference standarda

    TABLE 2

    • ↵a CC, critical concentration; BP, MIC breakpoint; NA, not applicable; CRS, composite reference standard. The CC is indicated for the agar proportion (AP) and MGIT960 methods; the BP is indicated for BMD frozen and BMD dry methods.

Back to top
Download PDF
Citation Tools
Print

Alerts
Email

KEYWORDS

Mycobacterium tuberculosis
bedaquiline
drug resistance
drug susceptibility testing
tuberculosis

Related Articles

Cited By...