DOI: 10.1128/AAC.01395-12
ABSTRACT
A biochemical test (Carba NP test II) was developed to identify carbapenemase production in Enterobacteriaceae and Pseudomonas spp., and to discriminate between the different types of carbapenemases (class A, B, and D). It is based on the detection of the acidification resulting from imipenem hydrolysis, coupled with tazobactam and EDTA as inhibitors. This is an easy and reliable technique (100% sensitivity and specificity) for detection and identification of carbapenemase types in Enterobacteriaceae and Pseudomonas aeruginosa.
FOOTNOTES
- Corresponding author : nordmann.patrice{at}bct.aphp.fr; Service de Bactériologie-Virologie, hôpital de Bicêtre 78 rue du Général Leclerc, 94275 K-Bicêtre, France, Tel : +33 1 45 21 36 32, Fax : +33 1 45 21 63 40
- Copyright © 2012, American Society for Microbiology. All Rights Reserved.
