Rapid identification of carbapenemase types using a biochemical test in Enterobacteriaceae and Pseudomonas

ABSTRACT

A biochemical test (Carba NP test II) was developed to identify carbapenemase production in Enterobacteriaceae and Pseudomonas spp., and to discriminate between the different types of carbapenemases (class A, B, and D). It is based on the detection of the acidification resulting from imipenem hydrolysis, coupled with tazobactam and EDTA as inhibitors. This is an easy and reliable technique (100% sensitivity and specificity) for detection and identification of carbapenemase types in Enterobacteriaceae and Pseudomonas aeruginosa.