PT - JOURNAL ARTICLE AU - Poirel, Laurent AU - Naas, Thierry AU - Guibert, Michele AU - Chaibi, El Bachir AU - Labia, Roger AU - Nordmann, Patrice TI - Molecular and Biochemical Characterization of VEB-1, a Novel Class A Extended-Spectrum β-Lactamase Encoded by an <em>Escherichia coli</em> Integron Gene AID - 10.1128/AAC.43.3.573 DP - 1999 Mar 01 TA - Antimicrobial Agents and Chemotherapy PG - 573--581 VI - 43 IP - 3 4099 - http://aac.asm.org/content/43/3/573.short 4100 - http://aac.asm.org/content/43/3/573.full SO - Antimicrob. Agents Chemother.1999 Mar 01; 43 AB - A clinical isolate, Escherichia coli MG-1, isolated from a 4-month-old Vietnamese orphan child, produced a β-lactamase conferring resistance to extended-spectrum cephalosporins and aztreonam. In a disk diffusion test, a typical synergistic effect between ceftazidime or aztreonam and clavulanic acid was observed along with an unusual synergy between cefoxitin and cefuroxime. The gene for VEB-1 (Vietnamese extended-spectrum β-lactamase) was cloned and expressed in E. coli JM109. The recombinant plasmid pRLT1 produced a β-lactamase with a pI of 5.35 and conferred high-level resistance to extended-spectrum (or oxyimino) cephalosporins and to aztreonam. V max values for extended-spectrum cephalosporins were uncommonly high, while the affinity of the enzyme for ceftazidime and aztreonam was relatively low. bla VEB-1 showed significant homology at the DNA level with only bla PER-1 andbla PER-2. Analysis of the deduced protein sequence showed that VEB-1 is a class A penicillinase having very low levels of homology with any other known β-lactamases. The highest percentage of amino acid identity was 38% with PER-1 or PER-2, two uncommon class A extended-spectrum enzymes. Exploration of the genetic environment of bla VEB-1 revealed the presence of gene cassette features, i.e., (i) a 59-base element associated with bla VEB-1; (ii) a second 59-base element just upstream of bla VEB-1, likely belonging to the aacA1-orfG gene cassette; (iii) two core sites (GTTRRRY) on both sides of bla VEB-1; and (iv) a second antibiotic resistance gene 3′ ofbla VEB-1, aadB. bla VEB-1 may therefore be the first class A extended-spectrum β-lactamase that is part of a gene cassette, which itself is likely to be located on a class 1 integron, as sulfamide resistance may indicate. Furthermore,bla VEB-1 is encoded on a large (&gt;100-kb) transferable plasmid found in a Klebsiella pneumoniae MG-2 isolated at the same time from the same patient, indicating a horizontal gene transfer.