PT  - JOURNAL ARTICLE
AU  - Eckert, C.
AU  - Gautier, V.
AU  - Saladin-Allard, M.
AU  - Hidri, N.
AU  - Verdet, C.
AU  - Ould-Hocine, Z.
AU  - Barnaud, G.
AU  - Delisle, F.
AU  - Rossier, A.
AU  - Lambert, T.
AU  - Philippon, A.
AU  - Arlet, G.
TI  - Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of <em>Enterobacteriaceae</em> in Paris, France
AID  - 10.1128/AAC.48.4.1249-1255.2004
DP  - 2004 Apr 01
TA  - Antimicrobial Agents and Chemotherapy
PG  - 1249--1255
VI  - 48
IP  - 4
4099  - http://aac.asm.org/content/48/4/1249.short
4100  - http://aac.asm.org/content/48/4/1249.full
SO  - Antimicrob. Agents Chemother.2004 Apr 01; 48
AB  - We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli isolates and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, from 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. The blaCTX-M genes encoding these β-lactamases were involved in this resistance, with a predominance of blaCTX-M-15. Ten of the 19 isolates produced both TEM-1- and CTX-M-type enzymes. One strain (E. coli TN13) expressed CMY-2, TEM-1, and CTX-M-14. blaCTX-M genes were found on large plasmids. In 15 cases the same insertion sequence, ISEcp1, was located upstream of the 5′ end of the blaCTX-M gene. In one case we identified an insertion sequence designated IS26. Examination of the other three blaCTX-M genes by cloning, sequencing, and PCR analysis revealed the presence of a complex sul1-type integron that includes open reading frame ORF513, which carries the bla gene and the surrounding DNA. Five isolates had the same plasmid DNA fingerprint, suggesting clonal dissemination of CTX-M-15-producing strains in the Paris area.