Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
Strains
E. coliDH10BF′ mcrAΔ(mrr-hsdRMS-mrcBC) Φ80ΔlacZDM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galK1 rpsL nupGGibco BRL
E. coliJM109endA1 hsdR17 gyrA96 Δ(lac proA)recA1 relA supE44 thi F′ (lacIqlacZΔM15 proAB+traD36)48
 Rifampin-resistant E. coli JM109 obtained in vitroRifampin-resistantThis study
C. meningosepticum PINTExtended-spectrum cephalosporin and carbapenem resistantThis study
C. meningosepticum AMAExtended-spectrum cephalosporin and carbapenem resistantThis study
C. meningosepticumGEOExtended-spectrum cephalosporin and carbapenem resistantThis study
C. meningosepticum CIP 6058Extended-spectrum cephalosporin and carbapenem resistantIPa
C. meningosepticum CIP 6059Extended-spectrum cephalosporin and carbapenem resistantIP
C. meningosepticum CIP 7830Extended-spectrum cephalosporin and carbapenem resistantIP
C. meningosepticum CIP 7905Extended-spectrum cephalosporin and carbapenem resistantIP
C. meningosepticum AB1572Extended-spectrum cephalosporin and carbapenem resistant7
C. meningosepticum H01, J100Extended-spectrum cephalosporin and carbapenem resistant7
C. indologenes CIP 101026Carbapenem resistantIP
C. indologenes PITCarbapenem resistantThis study
S. multivorum CIP 103686Carbapenem resistantIP
M. odoratus CIP 103105Carbapenem resistantIP
Plasmids
 pBK-CMV phagemidNeomycin and kanamycin resistant Stratagene
 pBR322Recombinant plasmid containing the 560-bpSspI-PstI internal fragment ofblaTEM-142
 pHUC37Recombinant plasmid containing the 435-bpPstI-NotI internal fragment ofblaSHV-324
 pPZ1Recombinant plasmid containing the 1.1-kbSnaBI fragment ofblaPER-125
 pRLT1Recombinant plasmid containing the 329-bpDraI-XmnI internal fragment ofblaVEB-132
 pPL1Recombinant plasmid containing the 396-bpEcoRI-PvuI internal fragment ofblaOXA-1829
 pBS11.9-kb Sau3AI fragment from C. meningosepticum PINT cloned into pBK-CMVThis study
  • a IP, Institut Pasteur, Paris, France.