Table 1.

Kanamycin resistance conferred to S. lividansTK21 by hybrid genes

PlasmidClassification of gene fragmentResistance level (μg/ml)a
0.5-kb BglII-BamHI1.3-kb BamHI
pANT3-1MutantMutant1,000
pANT201MutantWild type1,000
pANT4-5Wild typeWild type50
pANT205Wild typeMutant50
pIJ702b <5
  • a Maximum growth allowance concentrations on yeast extract-malt extract agar (Difco) plate were determined after incubation at 27°C for 3 days.

  • b Vector plasmid.