Table 2.

Multiplex PCR assays

Multiplex no.aPCR primer pairs combined (amplicon sizes)
1S. aureus (108 bp) + S. epidermidis (125 bp) +mecA (174 bp)
2S. aureus (108 bp) +S. epidermidis (125 bp) +aac(6′)-aph(2") (174 bp)
3S. aureus (108 bp) + S. epidermidis (125 bp) +ermA (139 bp)
4S. aureus (108 bp) +S. epidermidis (125 bp) + ermB (142 bp)
5S. aureus (108 bp) + S. epidermidis (125 bp) + ermC (190 bp)
6S. aureus (108 bp) +S. epidermidis (125 bp) + msrA (163 bp)
7S. aureus (108 bp) + S. epidermidis (125 bp) + blaZ (173 bp)
  • a All multiplex PCR assays included an internal control (241 bp).