Table 2.

MICs for E. coli mutants

HostPlasmidStatusaMIC (μg/ml)b
ABPCCERCTMLMOXCTXCAZCPDXCFPMAZTIPM
ML4947 (AmpD wild type)pKU403Wild type1612820.1250.1250.2510.030.060.5
pKU404H-mut>128>128>12883264>1280.25321
pKU405L-mut>128>128>12841632>1280.125162
pKU406I-mut>128>128>1282881280.0642
pKU407I-mut>128>128>1282881280.0642
pKU408pKU403 ΔampR 81620.1250.1250.2520.030.1250.5
pKU409pKU404 ΔampR 161640.1250.1250.2520.030.1250.5
pKU410pKU405 ΔampR 8820.1250.1250.2520.030.060.25
pKU414pKU406 ΔampR 81640.1250.1250.2520.030.1250.25
pKU411pKU403 ΔampR 820.1250.1250.060.1250.50.030.060.25
pKU412pKU404 ΔampC 420.250.1250.060.1250.50.030.060.25
pKU413pKU405 ΔampC 840.50.1250.1250.12510.030.060.25
pKU415pKU406 ΔampC 840.250.1250.1250.1250.50.030.060.25
pMW218Vector840.250.060.060.1250.50.030.060.25
820.250.1250.060.1250.50.030.060.25
ML4953 (AmpD mutant)pKU403Wild type>128>12812820.50.5320.030.251
pKU404H-mut>128>128>12883232>1280.25161
pKU405L-mut>128>128>12841616>1280.12581
pKU406I-mut>128>128>128488>1280.0681
pKU407I-mut>128>128>128488>1280.0682
pKU408pKU403 ΔampR 8810.1250.1250.12520.030.061
pKU409pKU404 ΔampR 81610.1250.1250.12520.030.1251
pKU410pKU405 ΔampR 880.50.1250.1250.2520.030.1251
pKU414pKU406 ΔampR 8810.1250.1250.2520.030.061
pKU411pKU403 ΔampC 840.250.1250.060.12510.030.061
pKU412pKU404 ΔampC 880.50.1250.060.12510.030.061
pKU413pKU405 ΔampC 880.250.1250.060.12510.030.031
pKU415pKU406 ΔampC 840.50.1250.060.12510.030.031
pMW218Vector440.250.1250.060.12510.030.061
440.250.1250.060.2510.030.061
  • a L-, I-, and H-mut, mutant plasmids with low, intermediate, and high levels of β-lactamase activity, respectively.

  • b ABPC, ampicillin; CER, cephaloridine; CTM, cefotiam; LMOX, latamoxef; CTX, cefotaxime; CAZ, ceftazidime; CPDX, cefpodoxime; CFPM, cefepime; AZT, aztreonam; IPM, imipenem.