Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource or reference
Strains
E. coli
  DH5α deoR supE44 Δ(lacZYA-argFV169) (φ80 dlacZΔM15) Fλ hsdR17 (rK mK +)recA1 endA1 gyrA96 thi-1 relA1 32
  JRG582Δ(ampDE)2 20
  STC172 ampD11E + 11
P. aeruginosaPAO1-DΔampD This study
Plasmids
 pEC1CCmr; ampC ampR from E. cloacae 30
 pBGS19+ Kmr; f1 Ori,lacPOZ 38
 pUCP24Gmr; Ori, Ori1600,lacZα 42
 pUCP26Tcr; Ori, Ori1600,lacZα 42
 pEX100TCbr; T Ori,lacZα 36
 pHUL4DE-2Kmr; ampDE region of P. aeruginosa PAO1 cloned into pBGS19+ 19
 pHUL4DE-3Gmr; ampDE region of P. aeruginosa PAO1 cloned into pEX100TThis study
 pHUL4DE-4Gmr; ampDE region of P. aeruginosa PAO1 containing the Gmr gene cloned into pEX100TThis study
 pHUL4DE26Kmr;ampDE region of P. aeruginosa PAO1 cloned into pUCP26This study
 pHUL4DE-M1Kmr;ampDE region of P. aeruginosa PAO4254 cloned into pBGS19+ This study
 pHUL4DE-M2Kmr;ampDE region of P. aeruginosaPs50SAI+ cloned into pBGS19+ This study
 pHUL4DE-M3Kmr; ampDE region ofP. aeruginosa Ps50SAI−con cloned into pBGS19+ This study
 pHUL4DE-M4Kmr;ampDE region of P. aeruginosa M1405 cloned into pBGS19+ This study
 pHUL4DE-M5Kmr;ampDE region of P. aeruginosa M2297P cloned into pBGS19+ This study
 pHUL4DE-M6Kmr;ampDE region of P. aeruginosaM2297−con cloned into pBGS19+ This study
  • a Cb, carbenicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Tc, tetracycline.