Table 1.

Characteristics of PZA-resistant clinical isolates ofM. tuberculosis

StrainaResistance to other drugscPZA MIC (μg/ml)PZase activitypncAmutation(s)b
Nucleotide change(s)Amino acid change(s)
9869INH, RMP, SM>300−11 promoter mutation A→G
10347Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9131Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
11041Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
11135Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9721Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9769Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
11823Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9132Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9579Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
10800Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
10350Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
11243Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9811Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9004Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
10348Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
10257Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
10003Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
11743Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
10611Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9155Monoresistant>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
9953INH, RMP, EMB>300C to A at 418, 8-bp deletion at 446Arg140→Ser, frameshift
W39117INH, RMP, EMB, Oflox>900A to G at 29Gln10→Arg
H3628INH, RMP>900A to C at 35Asp12→Ala
K10451NT>500C to A at 137Ala46→Glu
K10437NT>500C to T at 137Ala46→Val
F29684INH, RMP, EMB>900C to T at 137Ala46→Val
W5457NT>900C to T at 137Ala46→Val
A7259INH, RMP, SM>900C to T at 137Ala46→Val
A7183INH, RMP, SM>900C to T at 137Ala46→Val
8989NT>900C to T at 137Ala46->Val
K10552NT>100A to C at 158Asp53→Ala
PZA-R9717Monoresistant, in vitro mutant>900G to A at 203Trp68→stop
A7157INH, RMP, SM>900T to G at 213His71→Glu
A7153INH, RMP, EMB, SM300A to G at 245His82→Arg
K10400NT>500T to G at 254Leu85→Arg
T63168INH, RMP, EMB, SM>900G to A at 289Gly97→Ser
M52997INH, RMP, EMB, SM>900G to A at 289Gly97→Ser
10426INH, RMP, SM>300C to T at 401Ala134→Val
K10551NT>500G to C at 413Cys138→Ser
K10428NT>500G to A at 415Val139→Met
W76757INH, RMP, EMB, SM>300A to C at 422Gln141→Pro
K10447NT>500T to G at 464Val155→Gly
099-3-CIP8RMP, Oflox300C to A at 503Thr168→Asn
10467INH, RMP, SM>300G insertion at 52Frameshift
11627SM>300A insertion at 193Frameshift
A7156INH, RMP, SM>900A insertion at 193Frameshift
K10429NT>500G deletion at 301Frameshift
K10452NT>500C deletion at 341Frameshift
H2374RMP>900G insertion at 420Frameshift
H1033INH, RMP, SM, Oflox>900G insertion at 420Frameshift
K10436NT>100GG insertion at 428Frameshift
T61823INH, RMP, SM>900GG insertion at 420, T to G at 385Val130→Gly
11552INH>300No mutation
M49586NT>900No mutation
F57636INH, RMP>900No mutation
9739NT300+No mutation
10274NT100+No mutation
11830NT100+No mutation
  • a Strain designations with only numbers indicate strains from Quebec, Canada Strain designations starting with K indicate strains from South Korea, and strain designations starting with other letters indicate U.S. strains.

  • b Asterisks indicate new mutations not reported in previous studies (4, 6, 7, 10, 14, 15).

  • c SM, streptomycin; Oflox, ofloxacin; NT, not tested.