Table 1.

Antimicrobial susceptibilities of the clinical isolate, transconjugant, recombinant clone, and parental strains by agar dilution

DrugaMIC (μg/ml) for strain, recombinant (pI):
E. coli R170, (7.0, 9.0)E. coli J53-2, X170 (9.0)E. coliDH5α(pCA3000) (9.0)E. coli J53-2 E. coli DH5α(pBGS18)
Cefotaxime>256128128<0.125<0.125
Cefotaxime-clavulanic acid6420.125<0.125<0.125
Ceftazidime>256>256640.125<0.125
Ceftazidime-clavulanic acid6440.50.125<0.125
Aztreonam>256>25664<0.125<0.125
Aztreonam-clavulanic acid3220.250<0.125<0.125
Cefpirome12882<0.125<0.125
Cefpirome-clavulanic acid160.250<0.125<0.125<0.125
Cefepime6441<0.125<0.125
Cefepime-clavulanic acid160.125<0.125<0.125<0.125
Imipenem110.50.50.5
  • a A fixed concentration of clavulanic acid (2 μg/ml) was used.