Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
Strains
E. coli
  DH10B araD139Δ(ara, leu)7697 deoR endA1 galK1 galU nupG recA1 rpsL F′-mcrAΔ(mrr-hsdRMS-mrcBC)Φ80dlacZΔM15 ΔlacX74 Gibco BRL, Paris, France
  JM109 endA1 gyrA96 hsdR17 Δ(lac proA) relA recA1 supE44 thi F′ (lacI q lacZΔM15proAB+ traD36) 17
  In vitro-obtained rifampin-resistant JM109Rifampin resistant 17
P. aeruginosa
  COL-1Carbapenem-resistant clinical isolateThis study
  MKAM 12Carbapenem-resistant clinical isolate producing IMP-1Y. Arakawa
  In vitro-obtained ciprofloxacin-resistant P. aeruginosa PU21Ciprofloxacin resistant ilv leu streptromycin resistant 17
Plasmids
 pNOR-200045-kb natural plasmid that encodedbla VIM-2 This study
 pBK-CMVNeomycin resistant kanamycin resistantStratagene, Inc. (Ozyme, Saint-Quentin-en-Yvelines, France)
 pNOR-2001Recombinant plasmid containing a 3,843-bp BamHI insert encodingbla VIM-2 into pBK-CMVThis study